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International Orthopaedics
Published in: International Orthopaedics 5/2019

01-05-2019 | Original Paper

Escherichia coli-derived BMP-2-absorbed β-TCP granules induce bone regeneration in rabbit critical-sized femoral segmental defects

Authors: Yu Kuroiwa, Takahiro Niikura, Sang Yang Lee, Keisuke Oe, Takashi Iwakura, Tomoaki Fukui, Tomoyuki Matsumoto, Takehiko Matsushita, Kotaro Nishida, Ryosuke Kuroda

Published in: International Orthopaedics | Issue 5/2019

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Abstract

Purpose

This study investigated whether Escherichia coli-derived bone morphogenetic protein (BMP)-2 (E-BMP-2) adsorbed onto β-tricalcium phosphate (β-TCP) granules can induce bone regeneration in critical-size femoral segmental defects in rabbits.

Methods

Bone defects 20 mm in size and stabilized with an external fixator were created in the femur of New Zealand white rabbits, which were divided into BMP-2 and control groups. E-BMP-2-loaded β-TCP granules were implanted into defects of the BMP-2 group, whereas defects in the controls were implanted with β-TCP granules alone. At 12 and 24 weeks after surgery, radiographs were obtained of the femurs and histological and biomechanical assessments of the defect area were performed. Bone regeneration was quantified using micro-computed tomography at 24 weeks.

Results

Radiographic and histologic analyses revealed bone regeneration in the BMP-2 group but not the control group; no fracturing of newly formed bone occurred when the external fixator was removed at 12 weeks. At 24 weeks, tissue mineral density, the ratio of bone volume to total volume, and volumetric bone mineral density of the callus were higher in the BMP-2 group than in control animals. In the former, ultimate stress, extrinsic stiffness, and failure energy measurements for the femurs were higher at 24 weeks than at 12 weeks.

Conclusion

E-BMP-2-loaded β-TCP granules can effectively promote bone regeneration in long bone defects.
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Metadata
Title
Escherichia coli-derived BMP-2-absorbed β-TCP granules induce bone regeneration in rabbit critical-sized femoral segmental defects
Authors
Yu Kuroiwa
Takahiro Niikura
Sang Yang Lee
Keisuke Oe
Takashi Iwakura
Tomoaki Fukui
Tomoyuki Matsumoto
Takehiko Matsushita
Kotaro Nishida
Ryosuke Kuroda
Publication date
01-05-2019
Publisher
Springer Berlin Heidelberg
Published in
International Orthopaedics / Issue 5/2019
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI
https://doi.org/10.1007/s00264-018-4079-4

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