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01-11-2013 | Original Paper

Influence of cryopreservation, cultivation time and patient’s age on gene expression in Hyalograft® C cartilage transplants

Authors: Christian Albrecht, Brigitte Tichy, Sylvia Nürnberger, Lukas Zak, Markus Johannes Handl, Stefan Marlovits, Silke Aldrian

Published in: International Orthopaedics | Issue 11/2013

Abstract

Purpose

Our aim was to evaluate the impact of cryopreservation, cultivation time and patient’s age on the expression of specific chondrogenic markers in Hyalograft® C transplants.

Methods

Gene expression of chondrocyte markers [collagen type I (COL1A1), COL2A1, aggrecan, versican, melanoma inhibitory activity (MIA) and interleukin (IL)-1β] was analysed in cartilage biopsies (n = 17) and Hyalograft® C transplant samples (non-cryopreserved = 78, cryopreserved = 13) by quantitative real-time polymerase chain reaction (PCR). Correlation analyses were performed to evaluate the influence of the above-named parameters on the level of gene expression.

Results

Cryopreservation of cells was found to decrease COL2A1 and MIA significantly (4.6-fold, p < 0.01 and 2-fold, p < 0.045, respectively). The duration of cryopreservation had no further influence on the expression of these factors. No correlation was detected between cultivation time (75 ± 31 days) and the expression level of any gene. Cartilage transplants from older patients (>35 years) exhibited a significantly higher IL-1β expression (3.7-fold, p < 0.039) than transplants from younger patients (≤35 years).

Conclusions

Our data demonstrate that cryopreservation has a profound impact on chondrocyte metabolic activity by decreasing the expression of COL2A1 and MIA in Hyalograft® C transplants, independent of the duration of cryopreservation.

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Title: Influence of cryopreservation, cultivation time and patient’s age on gene expression in Hyalograft® C cartilage transplants
Authors: Christian Albrecht
Brigitte Tichy
Sylvia Nürnberger
Lukas Zak
Markus Johannes Handl
Stefan Marlovits
Silke Aldrian
Publication date: 01-11-2013
Publisher: Springer Berlin Heidelberg
Published in: International Orthopaedics / Issue 11/2013
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-013-2009-z

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Influence of cryopreservation, cultivation time and patient’s age on gene expression in Hyalograft® C cartilage transplants

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

Purpose

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