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International Orthopaedics
Published in: International Orthopaedics 1/2013

01-01-2013 | Original Paper

Comparison between normal and loose fragment chondrocytes in proliferation and redifferentiation potential

Authors: Kenichiro Sakata, Takayuki Furumatsu, Shinichi Miyazawa, Yukimasa Okada, Masataka Fujii, Toshifumi Ozaki

Published in: International Orthopaedics | Issue 1/2013

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Abstract

Purpose

Loose fragments in osteochondritis dissecans (OCD) of the knee require internal fixation. On the other hand, loose fragments derived from spontaneous osteonecrosis of the knee (SONK) are usually removed. However, the difference in healing potential between OCD- and SONK-related loose fragments has not been elucidated. In this study, we investigated proliferative activity and redifferentiation potential of normal cartilage-derived and loose fragment-derived chondrocytes.

Methods

Cells were prepared from normal articular cartilages and loose fragment cartilages derived from knee OCD and SONK. Cellular proliferation was compared. Redifferentiation ability of pellet-cultured chondrocytes was assessed by real-time PCR analyses. Mesenchymal differentiation potential was investigated by histological analyses. Positive ratio of a stem cell marker CD166 was evaluated in each cartilaginous tissue.

Results

Normal and OCD chondrocytes showed a higher proliferative activity than SONK chondrocytes. Chondrogenic pellets derived from normal and OCD chondrocytes produced a larger amount of safranin O-stained proteoglycans compared with SONK-derived pellets. Expression of chondrogenic marker genes was inferior in SONK pellets. The CD166-positive ratio was higher in normal cartilages and OCD loose fragments than in SONK loose fragments.

Conclusions

The OCD chondrocytes maintained higher proliferative activity and redifferentiation potential compared with SONK chondrocytes. Our results suggest that chondrogenic properties of loose fragment-derived cells and the amount of CD166-positive cells may affect the repair process of osteochondral defects.
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Metadata
Title
Comparison between normal and loose fragment chondrocytes in proliferation and redifferentiation potential
Authors
Kenichiro Sakata
Takayuki Furumatsu
Shinichi Miyazawa
Yukimasa Okada
Masataka Fujii
Toshifumi Ozaki
Publication date
01-01-2013
Publisher
Springer-Verlag
Published in
International Orthopaedics / Issue 1/2013
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI
https://doi.org/10.1007/s00264-012-1728-x

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