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International Orthopaedics
Published in: International Orthopaedics 5/2009

01-10-2009 | Original Paper

Biomechanical and histological comparison of the influence of oestrogen deficient state on tendon healing potential in rats

Authors: Esra Circi, Sercan Akpinar, Cenk Balcik, Didem Bacanli, Gulnur Guven, Rahmi Can Akgun, Ismail Cengiz Tuncay

Published in: International Orthopaedics | Issue 5/2009

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Abstract

Thirty-six female Sprague-Dawley rats were divided into two groups: oophrectomised (oestrogen deficient) rats and sham operated (oestrogen maintained) rats. Rats were sacrificed at six, ten, and 14 weeks. The rats were randomly chosen to have biomechanical evaluation on one side and histological evaluation on the other. Biomechanical testing was performed on an Instron machine to measure peak load. Histological sections were evaluated for cell proliferation, collagen-fibre organisation, fibroblast density, angiogenesis, inflammatory cells, chondroid and osseous metaplasia. Compared with the sham operated group, the oophrectomised group showed a lesser average maximum stress (42.9 N/m2 versus 33.7 N/m2) at six weeks, which was significant (p < .05). Succeeding weeks showed no significant biomechanical differences between the two groups. The sham operated group showed greater inflammatory response, which was statistically significant (p < 0.05), and also revealed greater cell proliferation and density. The results of this study revealed that endogenous oestrogen may improve healing of the Achilles tendon in rats.
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Metadata
Title
Biomechanical and histological comparison of the influence of oestrogen deficient state on tendon healing potential in rats
Authors
Esra Circi
Sercan Akpinar
Cenk Balcik
Didem Bacanli
Gulnur Guven
Rahmi Can Akgun
Ismail Cengiz Tuncay
Publication date
01-10-2009
Publisher
Springer-Verlag
Published in
International Orthopaedics / Issue 5/2009
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI
https://doi.org/10.1007/s00264-009-0778-1

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