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01-01-2010 | Original Paper

Immunohistochemical study of angiogenesis after local administration of platelet-rich plasma in a patellar tendon defect

Authors: Dimitris Lyras, Kostas Kazakos, Dionysios Verettas, Alexandros Polychronidis, Constantinos Simopoulos, Sotirios Botaitis, George Agrogiannis, Anna Kokka, Efstratios Patsouris

Published in: International Orthopaedics | Issue 1/2010

Abstract

A full thickness defect was made in the central portion of the patellar tendon of 48 New Zealand white rabbits. Platelet-rich plasma (PRP) gel was then applied and filled the tendon defect. The same procedure was performed in the control group, without the application of PRP. Animals were sacrificed after one, two, three, and four weeks. Histological and immunohistochemical analyses using a monoclonal antibody against CD31 were performed. The histological examination showed a superior healing process in the PRP group compared with the control group. Especially in the third week, the tissue formed in the PRP group was more mature and dense with less elastic fibres remaining. Neovascularisation was significantly higher in the PRP group during the first two weeks and significantly lower in the third and fourth weeks (p < 0.0001). Histological examination and study of angiogenesis showed that the application of PRP enhances and accelerates the tendon healing process.

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Title: Immunohistochemical study of angiogenesis after local administration of platelet-rich plasma in a patellar tendon defect
Authors: Dimitris Lyras
Kostas Kazakos
Dionysios Verettas
Alexandros Polychronidis
Constantinos Simopoulos
Sotirios Botaitis
George Agrogiannis
Anna Kokka
Efstratios Patsouris
Publication date: 01-01-2010
Publisher: Springer-Verlag
Published in: International Orthopaedics / Issue 1/2010
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-009-0728-y

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Immunohistochemical study of angiogenesis after local administration of platelet-rich plasma in a patellar tendon defect

Abstract

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Abstract

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