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International Orthopaedics
Published in: International Orthopaedics 1/2006

01-02-2006 | Original paper

Changes in biochemical parameters after anterior cruciate ligament injury

Authors: H. Higuchi, K. Shirakura, M. Kimura, M. Terauchi, T. Shinozaki, H. Watanabe, K. Takagishi

Published in: International Orthopaedics | Issue 1/2006

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Abstract

We studied the biochemical characteristics of human knees with deficient anterior cruciate ligaments (ACL) and analysed their relationship to the time after ligamentous injury. Thirty-two patients with isolated ACL-injured knees and six healthy volunteers were enrolled. Synovial fluid samples were centrifuged after aspiration during arthroscopic examination, and aliquots of supernatant were frozen and stored at −80°C. The samples were analysed for interleukin (IL)-1β, tumour necrosis factor (TNF)-α, IL-6, matrix metalloproteinase (MMP)-3, and tissue inhibitor of metalloproteinase (TIMP)-1 using commercially available sandwich enzyme-linked immunosorbent assay. In fluid from ACL-injured knees, the average concentrations of IL-6, MMP-3 and TIMP-1 were highly elevated in comparison with normal controls. There was a statistically significant correlation between the concentrations of MMP-3 and IL-6. The IL-6 and TIMP-1 concentrations were interrelated. The concentration of MMP-3 remained high, independent of the duration since the injury, whereas the TIMP-1 and IL-6 levels decreased. The results suggest that the timing of the treatment of an ACL-injured knee might be of importance.
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Metadata
Title
Changes in biochemical parameters after anterior cruciate ligament injury
Authors
H. Higuchi
K. Shirakura
M. Kimura
M. Terauchi
T. Shinozaki
H. Watanabe
K. Takagishi
Publication date
01-02-2006
Publisher
Springer-Verlag
Published in
International Orthopaedics / Issue 1/2006
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI
https://doi.org/10.1007/s00264-005-0023-5

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