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International Orthopaedics
Published in: International Orthopaedics 1/2005

01-02-2005 | Original Paper

Hypoxia and HIF-1α in osteoarthritis

Authors: David Pfander, Thorsten Cramer, Bernd Swoboda

Published in: International Orthopaedics | Issue 1/2005

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Abstract

We have previously shown that functional inactivation of hypoxia-inducible factor-1α (HIF-1α) in growth-plate chondrocytes will dramatically inhibit anaerobic energy generation and matrix synthesis. Using immunohistochemistry, we have now analyzed the spatial distribution of HIF-1α and its target genes in normal cartilage and in cartilage from knee joints with osteoarthritis. We detected HIF-1α and its target genes in both types of cartilage. In cartilage from joints with osteoarthritis, the number of HIF-1α-, Glut-1-, and PGK-1-stained chondrocytes increased with the severity of osteoarthritis. Activated matrix synthesis and strongly decreased oxygen levels are hallmarks of osteoarthritic cartilage. Thus, we assume that chondrocytes are depending on the adaptive functions of HIF-1α in order to maintain ATP levels and thereby matrix synthesis during the course of osteoarthritis.
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Metadata
Title
Hypoxia and HIF-1α in osteoarthritis
Authors
David Pfander
Thorsten Cramer
Bernd Swoboda
Publication date
01-02-2005
Publisher
Springer-Verlag
Published in
International Orthopaedics / Issue 1/2005
Print ISSN: 0341-2695
Electronic ISSN: 1432-5195
DOI
https://doi.org/10.1007/s00264-004-0618-2

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