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Cancer Immunology, Immunotherapy
Published in: Cancer Immunology, Immunotherapy 11/2009

01-11-2009 | Original article

Dynamic changes of interleukin-8 network along the colorectal adenoma–carcinoma sequence

Authors: Guanglin Cui, Aping Yuan, Rasmus Goll, Barthold Vonen, Jon Florholmen

Published in: Cancer Immunology, Immunotherapy | Issue 11/2009

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Abstract

The interleukin-8 (IL-8) network is involved in the colorectal cancer (CRC) progression. However, its role during the adenoma–carcinoma transition to date has not been fully investigated. To evaluate the dynamic changes of IL-8 network along the colorectal adenoma–carcinoma sequence, we examined the tissue IL-8 mRNA level in colorectal biopsies from 53 colorectal adenomas, 44 CRCs and 18 controls by quantitative real-time PCR (Q-PCR), and the expressions of IL-8 and its receptors (IL-8RA and IL-8RB) in the tumor microenvironment by immunohistochemistry (IHC) and double IHCs. The results showed that the tissue IL-8 mRNA level began to increase in the precancerous lesions (adenomas) as compared with the controls and became even higher in the CRCs. Significantly, the increase of IL-8 mRNA levels was associated with the increase of dysplastic grades in the adenomas, and also paralleled to the increase of Duke’s stages in the CRCs. IHC results revealed that IL-8 and its receptors, IL-8RA and IL-8RB, were observed both in the stroma and in the adenomatous/cancerous cells. By double IHCs, the IL-8 expression was characterized in macrophages, lymphocytes and myofibroblasts in the tumor stroma. Further double IHC identified the co-expression of IL-8 receptors (IL-8RA and IL-8RB) with CD34 positive tumor-associated microvessels in both the adenomas and CRCs. We, therefore, conclude that activated IL-8 network in the tumor microenvironment may function as a significant regulatory factor for the adenoma progression and the adenoma–carcinoma transition.
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Metadata
Title
Dynamic changes of interleukin-8 network along the colorectal adenoma–carcinoma sequence
Authors
Guanglin Cui
Aping Yuan
Rasmus Goll
Barthold Vonen
Jon Florholmen
Publication date
01-11-2009
Publisher
Springer-Verlag
Published in
Cancer Immunology, Immunotherapy / Issue 11/2009
Print ISSN: 0340-7004
Electronic ISSN: 1432-0851
DOI
https://doi.org/10.1007/s00262-009-0702-y

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