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01-03-2004 | Review

Tetraspanins connect several types of Ig proteins: IgM is a novel component of the tetraspanin web on B-lymphoid cells

Authors: François Le Naour, Stéphanie Charrin, Valérie Labas, Jean-Pierre Le Caer, Claude Boucheix, Eric Rubinstein

Published in: Cancer Immunology, Immunotherapy | Issue 3/2004

Abstract

The tetraspanins form a family of about 30 molecules mainly expressed on the cell surface. They have been reported to be involved in many physiological or pathological processes, such as fertilization, immune response, development of the nervous system, and metastasis, as well as in infectious diseases (HCV, malaria, etc.). The tetraspanins may play a role as “organizers” of multimolecular complexes on the cell surface associating numerous proteins, the “tetraspanin web.” To better define the composition of the tetraspanin web, its characterization has been recently performed using mass spectrometry and proteomics. We report the proteomic analysis of tetraspanin complexes on B-lymphoid cells. Immunoprecipitation experiments were performed using mAbs directed against the tetraspanin CD9, and associated molecules were identified by MALDI-TOF (matrix assisted laser desorption ionization time of flight) mass spectrometry. This led to the identification of IgM as a novel component of the complexes. Thus, tetraspanins may connect several types of proteins with Ig domains, including HLA-DR, EWI-2, and IgM, that may play a role in immune responses.

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Title: Tetraspanins connect several types of Ig proteins: IgM is a novel component of the tetraspanin web on B-lymphoid cells
Authors: François Le Naour
Stéphanie Charrin
Valérie Labas
Jean-Pierre Le Caer
Claude Boucheix
Eric Rubinstein
Publication date: 01-03-2004
Publisher: Springer-Verlag
Published in: Cancer Immunology, Immunotherapy / Issue 3/2004
Print ISSN: 0340-7004
Electronic ISSN: 1432-0851
DOI: https://doi.org/10.1007/s00262-003-0477-5

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