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European Journal of Nuclear Medicine and Molecular Imaging

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01-10-2010 | Original Article

PET imaging of inflammation and adenocarcinoma xenografts using vascular adhesion protein 1 targeting peptide ⁶⁸Ga-DOTAVAP-P1: comparison with ¹⁸F-FDG

Authors: Anu Autio, Tiina Ujula, Pauliina Luoto, Satu Salomäki, Sirpa Jalkanen, Anne Roivainen

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Issue 10/2010

Abstract

Purpose

The aim of this study was to evaluate inflammation and tumour imaging with a vascular adhesion protein 1 (VAP-1) targeting peptide ⁶⁸Ga-DOTAVAP-P1 in comparison with ¹⁸F-FDG.

Methods

Rats with both subcutaneous human pancreatic adenocarcinoma xenografts and turpentine oil-induced acute sterile inflammation were evaluated by dynamic positron emission tomography (PET) and by digital autoradiography of tissue cryosections. Subsequently, the autoradiographs were combined with histological and immunohistological analysis of the sections.

Results

⁶⁸Ga-DOTAVAP-P1 delineated acute, sterile inflammation comparable with ¹⁸F-FDG. However, the tumour uptake of ⁶⁸Ga-DOTAVAP-P1 was low in contrast to prominent ¹⁸F-FDG uptake. The standardised uptake values of inflammation and tumours by PET were 1.1 ± 0.4 (mean ± SEM) and 0.4 ± 0.1 for ⁶⁸Ga-DOTAVAP-P1 and 2.0 ± 0.5 and 1.6 ± 0.8 for ¹⁸F-FDG, respectively. In addition, PET studies showed inflammation to muscle and tumour to muscle ratios of 5.1 ± 3.1 and 1.7 ± 0.3 for ⁶⁸Ga-DOTAVAP-P1 and 6.2 ± 0.7 and 4.6 ± 2.2 for ¹⁸F-FDG, respectively. Immunohistochemistry revealed increased expression of luminal VAP-1 on the endothelium at the site of inflammation and low expression in the tumour

Conclusion

The ⁶⁸Ga-DOTAVAP-P1 PET was able to visualise inflammation better than tumour, which was in accordance with the luminal expression of VAP-1 on vasculature in these experimental models.

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Title: PET imaging of inflammation and adenocarcinoma xenografts using vascular adhesion protein 1 targeting peptide 68Ga-DOTAVAP-P1: comparison with 18F-FDG
Authors: Anu Autio
Tiina Ujula
Pauliina Luoto
Satu Salomäki
Sirpa Jalkanen
Anne Roivainen
Publication date: 01-10-2010
Publisher: Springer-Verlag
Published in: European Journal of Nuclear Medicine and Molecular Imaging / Issue 10/2010
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-010-1497-y

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

PET imaging of inflammation and adenocarcinoma xenografts using vascular adhesion protein 1 targeting peptide ⁶⁸Ga-DOTAVAP-P1: comparison with ¹⁸F-FDG

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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