Skip to main content
Top
Published in: Urolithiasis 4/2005

01-08-2005 | Original Paper

Effects of trace metals on the inhibition of calcium oxalate crystallization

Authors: J. A. Muñoz, M. Valiente

Published in: Urolithiasis | Issue 4/2005

Login to get access

Abstract

The aim of this study was to examine the possible effects of some trace metals on the inhibition of calcium oxalate crystallization. A test of urinary lithogenic risk was used to follow the crystallization of calcium oxalate from artificial urine in the presence of several metal ions assayed in their physiological concentrations. Interactions of these metal ions with known inhibitors of such crystallization (phytate, pyrophosphate, citrate and chondroitin sulphate) were also investigated. None of the metals affected the inhibition of calcium oxalate crystallization at concentrations approximating those found in normal urine, with the exception of the Fe3+ ions. Interactions of Fe3+ with some urinary components produced both synergic (phytate and pyrophosphate) and negative (citrate) effects on preventing crystallization. These effects are explained in terms of the affinity of the inhibitors for the calcium oxalate crystal surface and their ability to form stable complexes in urine. Because of the minimal concentrations, we conclude that physiological concentrations of trace elements in urine have no significant influence on calcium oxalate crystallization. In this sense, ferric ions, which exhibit an intrinsic high inhibitory capacity of calcium oxalate crystallization at physiological concentrations, even increased by the concomitant presence of phytate and pyrophosphate, are probably unable to act as powerful inhibitors in the presence of physiological urinary concentrations of citrate, due to the formation of highly stable complexes in solution without inhibitory activity.
Literature
1.
go back to reference Grases F, Costa-Bauzá A, García-Ferragut L (1998) Biopathological crystallization: a general view about the mechanisms of renal stone formation. Adv Coll Interf Sci 74: 169CrossRef Grases F, Costa-Bauzá A, García-Ferragut L (1998) Biopathological crystallization: a general view about the mechanisms of renal stone formation. Adv Coll Interf Sci 74: 169CrossRef
2.
go back to reference Robertson WG, Peacock M (1985) Pathogenesis of urolithiasis. In: Scheneider HJ, Peacock M, Robertson WG, Vahlensieck W (eds) Urolithiasis: etiology, diagnosis. Springer Verlag, Heidelberg New York, p 185 Robertson WG, Peacock M (1985) Pathogenesis of urolithiasis. In: Scheneider HJ, Peacock M, Robertson WG, Vahlensieck W (eds) Urolithiasis: etiology, diagnosis. Springer Verlag, Heidelberg New York, p 185
3.
go back to reference Fleisch H (1978) Inhibitors and promoters of stone formation. Kidney Int 13: 361PubMed Fleisch H (1978) Inhibitors and promoters of stone formation. Kidney Int 13: 361PubMed
4.
go back to reference Ryall RL, Harnett RM, Marshal VR (1981) The effect of urine, pyrophosphate, citrate, magnesium and glycosaminoglycans on the growth and aggregation of calcium oxalate crystals in vitro. Clin Chim Acta 112: 349PubMed Ryall RL, Harnett RM, Marshal VR (1981) The effect of urine, pyrophosphate, citrate, magnesium and glycosaminoglycans on the growth and aggregation of calcium oxalate crystals in vitro. Clin Chim Acta 112: 349PubMed
5.
go back to reference Grases F, García-Ferragut L, Costa-Bauzá, March JG (1996) Study of the effects of different substances on the early stages of papillary stone formation. Nephron 73: 561PubMed Grases F, García-Ferragut L, Costa-Bauzá, March JG (1996) Study of the effects of different substances on the early stages of papillary stone formation. Nephron 73: 561PubMed
6.
go back to reference Meyer JL, Smith LH (1975) Growth of calcium oxalate crystals. II. Inhibition by natural crystal growth inhibitors. Invest Urol 13: 36PubMed Meyer JL, Smith LH (1975) Growth of calcium oxalate crystals. II. Inhibition by natural crystal growth inhibitors. Invest Urol 13: 36PubMed
7.
go back to reference Hofbauer J, Steffan I, Höbarth K, Vujicic G, Schwetz H, Reich G (1991) Trace elements and urinary stone formation: new aspects of the pathological mechanism of urinary stone formation. J Urol 145: 93PubMed Hofbauer J, Steffan I, Höbarth K, Vujicic G, Schwetz H, Reich G (1991) Trace elements and urinary stone formation: new aspects of the pathological mechanism of urinary stone formation. J Urol 145: 93PubMed
8.
go back to reference Elliot JS, Ribeiro ME (1973) The urinary excretion of trace metals in patients with calcium oxalate urinary stone. Invest Urol 10: 253PubMed Elliot JS, Ribeiro ME (1973) The urinary excretion of trace metals in patients with calcium oxalate urinary stone. Invest Urol 10: 253PubMed
9.
go back to reference Sutor DJ (1969) Growth studies of calcium oxalate in the presence of various ions and ions. Brit J Urol 41: 171PubMed Sutor DJ (1969) Growth studies of calcium oxalate in the presence of various ions and ions. Brit J Urol 41: 171PubMed
10.
go back to reference Elliot JS, Eusebio E (1967) Calcium oxalate solubility: the effect of trace metals. Invest Urol 4: 428PubMed Elliot JS, Eusebio E (1967) Calcium oxalate solubility: the effect of trace metals. Invest Urol 4: 428PubMed
11.
go back to reference Eusebio E, Elliot JS (1967) Effect of trace metals on the crystallization of calcium oxalate. Invest Urol 4: 431PubMed Eusebio E, Elliot JS (1967) Effect of trace metals on the crystallization of calcium oxalate. Invest Urol 4: 431PubMed
12.
go back to reference Welshman SG, McGeown MG (1972) A quantitative investigation of the effects of the growth of calcium oxalate crystals on potential inhibitors. Br J Urol 44: 67 Welshman SG, McGeown MG (1972) A quantitative investigation of the effects of the growth of calcium oxalate crystals on potential inhibitors. Br J Urol 44: 67
13.
go back to reference Meyer JL, Angino EE (1977) The role of trace metals in calcium urolithiasis. Invest Urol 14: 347PubMed Meyer JL, Angino EE (1977) The role of trace metals in calcium urolithiasis. Invest Urol 14: 347PubMed
14.
go back to reference Wolf JS, Stoller ML (1994) Inhibition of calculi fragment growth by metal-bisphosphonate complexes demonstrated with a new assay measuring the surface activity of urolithiasis inhibitors. J Urol 152: 1609PubMed Wolf JS, Stoller ML (1994) Inhibition of calculi fragment growth by metal-bisphosphonate complexes demonstrated with a new assay measuring the surface activity of urolithiasis inhibitors. J Urol 152: 1609PubMed
15.
go back to reference Meyer JL, Thomas WC. (1982) Trace metal-citric acid complexes as inhibitors of calcification and crystal growth. II. Efects of Fe(III), Cr(III) and Al(III) complexes on calicum oxalate crystal growth. J Urol 128: 1376PubMed Meyer JL, Thomas WC. (1982) Trace metal-citric acid complexes as inhibitors of calcification and crystal growth. II. Efects of Fe(III), Cr(III) and Al(III) complexes on calicum oxalate crystal growth. J Urol 128: 1376PubMed
16.
go back to reference Baumann JM (1988) How to measure crystallization conditions in urine: a comparison of 7 methods. Urol Res 16: 137PubMed Baumann JM (1988) How to measure crystallization conditions in urine: a comparison of 7 methods. Urol Res 16: 137PubMed
17.
go back to reference Kavanagh JP (1992) Methods for the study of calcium oxalate crystallization and their application to urolithiasis research. Scanning Microsc 6: 685PubMed Kavanagh JP (1992) Methods for the study of calcium oxalate crystallization and their application to urolithiasis research. Scanning Microsc 6: 685PubMed
18.
go back to reference Grases F, García-Ferragut L, Costa-Bauzá A, Conte A, García-Raja A (1997) Simple test to evaluate the risk of urinary calcium stone formation. Clin Chim Acta 263: 43PubMed Grases F, García-Ferragut L, Costa-Bauzá A, Conte A, García-Raja A (1997) Simple test to evaluate the risk of urinary calcium stone formation. Clin Chim Acta 263: 43PubMed
19.
go back to reference Grases F, Söhnel O, Costa-Bauzá A, Ramis M, Wang Z (2001) Study on concretions developed around urinary catheters and mechanisms of renal calculi development. Nephron 88: 320PubMed Grases F, Söhnel O, Costa-Bauzá A, Ramis M, Wang Z (2001) Study on concretions developed around urinary catheters and mechanisms of renal calculi development. Nephron 88: 320PubMed
20.
go back to reference Komaromy-Hiller G, Ash KO, Costa R, Howerton K (2000) Comparison of representative ranges based on US patient populations and literature reference intervals for urinary trace elements. Clin Chim Acta 296: 71PubMed Komaromy-Hiller G, Ash KO, Costa R, Howerton K (2000) Comparison of representative ranges based on US patient populations and literature reference intervals for urinary trace elements. Clin Chim Acta 296: 71PubMed
21.
go back to reference Caroli S, Alimonti A, Coni E, Petrucci F, Senofonte O, Violante N (1994) The assessment of reference values for elements in human biological tissues and fluids. A systematic review. Crit Rev Anal Chem 24: 363 Caroli S, Alimonti A, Coni E, Petrucci F, Senofonte O, Violante N (1994) The assessment of reference values for elements in human biological tissues and fluids. A systematic review. Crit Rev Anal Chem 24: 363
22.
go back to reference Wandt MAE, Pougnet MAB (1986) Simultaneous determination of major and trace elements in urinary calculi by microwave-assisted digestion and inductively coupled plasma atomic emission spectrometric analysis. Analyst 111: 1249PubMed Wandt MAE, Pougnet MAB (1986) Simultaneous determination of major and trace elements in urinary calculi by microwave-assisted digestion and inductively coupled plasma atomic emission spectrometric analysis. Analyst 111: 1249PubMed
23.
go back to reference Levinson AA, Nosal M, Davidman M, Prien EL, Stenvenson RG (1978) Trace elements in kidney stones from three areas in the united states. Invest Urol 15: 270PubMed Levinson AA, Nosal M, Davidman M, Prien EL, Stenvenson RG (1978) Trace elements in kidney stones from three areas in the united states. Invest Urol 15: 270PubMed
24.
go back to reference Durak I, Kilic Z, Sahin A, Akpoyraz M (1992) Analysis of calcium, iron, copper and zinc contents of nucleus and crust parts of urinary calculi. Urol Res 20: 23PubMed Durak I, Kilic Z, Sahin A, Akpoyraz M (1992) Analysis of calcium, iron, copper and zinc contents of nucleus and crust parts of urinary calculi. Urol Res 20: 23PubMed
25.
go back to reference Wandt MAE, Underhill LG (1988) Covariance biplot analysis of trace element concentrations in urinary stones. Br J Urol 61: 474PubMed Wandt MAE, Underhill LG (1988) Covariance biplot analysis of trace element concentrations in urinary stones. Br J Urol 61: 474PubMed
26.
go back to reference Marthel AE, Smith RM (1974) Critical stability constants. Plenum Press, New York Marthel AE, Smith RM (1974) Critical stability constants. Plenum Press, New York
27.
go back to reference Isao Y (1994) A spectrophotometric study on the mixed-ligands complexes of Iron(III)-N-(o-hydroxybenzyl)iminodiacetate with several anions. Kenkyu Hokoku—Kumamoto Kogyo Daigaku 19: 147 Isao Y (1994) A spectrophotometric study on the mixed-ligands complexes of Iron(III)-N-(o-hydroxybenzyl)iminodiacetate with several anions. Kenkyu Hokoku—Kumamoto Kogyo Daigaku 19: 147
Metadata
Title
Effects of trace metals on the inhibition of calcium oxalate crystallization
Authors
J. A. Muñoz
M. Valiente
Publication date
01-08-2005
Publisher
Springer-Verlag
Published in
Urolithiasis / Issue 4/2005
Print ISSN: 2194-7228
Electronic ISSN: 2194-7236
DOI
https://doi.org/10.1007/s00240-005-0468-4

Other articles of this Issue 4/2005

Urolithiasis 4/2005 Go to the issue