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Published in: Urolithiasis 5/2004

01-10-2004 | Original Paper

Raxofelast, a hydrophilic vitamin e-like antioxidant, reduces testicular ischemia-reperfusion injury

Authors: Carmelo Romeo, Pietro Antonuccio, Maria Esposito, Herbert Marini, Pietro Impellizzeri, Nunzio Turiaco, Domenica Altavilla, Alessandra Bitto, Biagio Zuccarello, Francesco Squadrito

Published in: Urolithiasis | Issue 5/2004

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Abstract

Testis torsion is a surgical emergency that lead to permanent gonad damage. The damage has been ascribed to mechanisms of ischemia-reperfusion similar to other tissues. The mechanisms involved are different, but the lipid peroxidation of plasma membrane, caused by reactive oxygen species (ROS), generated particularly during reperfusion, is one of the most accredited. In the present study, we aimed to evaluate the effects of raxofelast, a vitamin E-like antioxidant with potent action and no systemic toxicity, on lipid peroxidation and histopathology in both testes after unilateral testicular torsion and detorsion. Adult male Wistar rats were subjected to total occlusion (3 h) of the left testis followed by 4 hours of reperfusion (TI/R). Sham testicular ischemia-reperfusion rats (SHAM TI/R) were used as controls. The animals were then randomized to receive either vehicle (1 ml/kg/i.p. of a dimetylsulphoxide/NaCl 0.9% 1:10 v/v solution, injected either 15 min before detorsion and 15 min after detorsion) or raxofelast (20 mg/kg i.p. 15 min before detorsion and 15 min after detorsion). Conjugated dienes (CD) levels, an index of lipid peroxidation, and testis histopathology were evaluated. Testicular ischemia reperfusion (TI/R) in untreated rats produced high testicular levels of CD (3.6±0.3 ΔABS/g protein on the left side and 2.5±0.2 ΔABS/g protein on the right side). Furthermore, histological examination revealed marked damage to the testis interstitium with severe haemorrhage and edema. The administration of raxofelast lowered CD levels (2.8±0.2 ΔABS/g protein on the left side and 1.9±0.1 ΔABS/g protein in the right side) and significantly reduced histological damage. These data suggest that the hydrophilic vitamin E-like antioxidants are good candidates for designing a novel therapeutic strategy to halt the oxidative stress that follows acute testis torsion.
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Metadata
Title
Raxofelast, a hydrophilic vitamin e-like antioxidant, reduces testicular ischemia-reperfusion injury
Authors
Carmelo Romeo
Pietro Antonuccio
Maria Esposito
Herbert Marini
Pietro Impellizzeri
Nunzio Turiaco
Domenica Altavilla
Alessandra Bitto
Biagio Zuccarello
Francesco Squadrito
Publication date
01-10-2004
Publisher
Springer-Verlag
Published in
Urolithiasis / Issue 5/2004
Print ISSN: 2194-7228
Electronic ISSN: 2194-7236
DOI
https://doi.org/10.1007/s00240-004-0436-4

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