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European Journal of Plastic Surgery
Published in: European Journal of Plastic Surgery 6/2020

01-12-2020 | Edema | Experimental Study

Effects of luteolin on random pattern skin flaps in rats

Author: Mehmet Sönmez

Published in: European Journal of Plastic Surgery | Issue 6/2020

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Abstract

Background

Luteolin, a natural flavonoid, has anti-inflammatory, antioxidant, and antiapoptotic properties. Studies showed that luteolin protects tissues against ischemia-reperfusion injury on liver, neural tissue, and myocard. This study was designed to evaluate the effects of luteolin on random pattern skin flap in rats.

Methods

Sixteen rats were randomly divided into two groups (n = 8 each group: luteolin group: Land control group: C). Ten milligram per kilogram of luteolin diluted with saline was given intraperitoneally 30 min before and 12 h after surgery. Tissue samples were taken for biochemical analysis at the first day and at the seventh day for histopathological analysis. Catalase, malondialdehyde (MDA), xanthine oxidase (XO), and nitric oxide (NO) levels were measured with spectrophotometrical method. Edema, inflammatory cell infiltration, necrosis, and fibrosis were evaluated and scored. Flap survival ratios were measured. Statistical analysis was performed by SPSS for Windows 15.0.

Results

Tissue catalase levels were found significantly higher and MDA levels significantly lower in luteolin group respectively (L 65.38, C 39.80, p = 0.02; L 3.525, C 6.97, p = 0.01). XO and NO levels were found higher in luteolin group, but this difference was not significant (L 0.089, C 0.078, p = 0.17; L 41.75, C 37, p = 0.79). Edema, inflammatory cell infiltration, and necrosis were scored significantly lower in luteolin group (L +, C +++, p = 0.003; L +, C +++, p = 0.0004; L ±, C +++, p = 0.0021). Fibrosis scores were significantly higher in luteolin group (L +++, C +, p < 0.001). Flap survival was significantly higher in luteolin group (L 37.55, C 49.7, p = 0.02).

Conclusions

Luteolin has shown beneficial effects on random pattern skin flap in rats. Additional experiments are warranted to investigate the XO and NO mechanisms during ischemic injury.
Level of evidence: Not ratable
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Metadata
Title
Effects of luteolin on random pattern skin flaps in rats
Author
Mehmet Sönmez
Publication date
01-12-2020
Publisher
Springer Berlin Heidelberg
Keyword
Edema
Published in
European Journal of Plastic Surgery / Issue 6/2020
Print ISSN: 0930-343X
Electronic ISSN: 1435-0130
DOI
https://doi.org/10.1007/s00238-020-01719-8

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