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European Journal of Plastic Surgery
Published in: European Journal of Plastic Surgery 4/2016

Open Access 01-08-2016 | Experimental Study

Negative pressure wound therapy induces early wound healing by increased and accelerated expression of vascular endothelial growth factor receptors

Authors: Tsuruhito Tanaka, Nirmal Panthee, Yoshifumi Itoda, Naoko Yamauchi, Masashi Fukayama, Minoru Ono

Published in: European Journal of Plastic Surgery | Issue 4/2016

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Abstract

Background

Negative pressure wound therapy (NPWT) is commonly used to accelerate wound healing, especially following thoracic surgery; however, the mechanism remains elusive. Given the important role of vasculogenesis in wound healing, we evaluated whether NPWT might accelerate vasculogenesis in the wound area. Toward this end, we investigated the temporal expression of vascular endothelial growth factor receptors (VEGFRs) in an NPWT-wound healing rabbit model.

Methods

Rabbits were divided into an NPWT group and a non-NPWT control group, and tissue samples were collected around wounds made in the skin of each rabbit at five time points: 0, 7, 14, 21, and 28 days after wound creation. Cryopreserved samples were then immunostained and subject to image analysis to evaluate the temporal changes in VEGFR1, VEGFR2, and VEGFR3 expression in the wound-healing process.

Results

Results of histological analysis of the temporal changes in VEGFR expression throughout the healing process showed that compared to the control group, VEGFR2 and VEGFR3 were abundantly and rapidly expressed in the NPWT group, and were expressed earlier than VEGFR1.

Conclusions

NPWT promotes the expression of VEGFR2 and VEGFR3, which provides insight into the mechanism by which NPWT accelerates wound healing.
Level of Evidence: Not ratable.
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Metadata
Title
Negative pressure wound therapy induces early wound healing by increased and accelerated expression of vascular endothelial growth factor receptors
Authors
Tsuruhito Tanaka
Nirmal Panthee
Yoshifumi Itoda
Naoko Yamauchi
Masashi Fukayama
Minoru Ono
Publication date
01-08-2016
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Plastic Surgery / Issue 4/2016
Print ISSN: 0930-343X
Electronic ISSN: 1435-0130
DOI
https://doi.org/10.1007/s00238-016-1200-z

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