Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Neuroradiology
Published in: Neuroradiology 11/2007

01-11-2007 | Diagnostic Neuroradiology

Evaluation of invasiveness of astrocytoma using 1H-magnetic resonance spectroscopy: correlation with expression of matrix metalloproteinase-2

Authors: Kai Zhang, Chuanfu Li, Ying Liu, Li Li, Xiangxing Ma, Xiangshui Meng, Dechao Feng

Published in: Neuroradiology | Issue 11/2007

Login to get access

Abstract

Introduction

Even low-grade astrocytomas infiltrate the entire brain, a feature that precludes their successful therapy. So to assess the invasive potential of astrocytoma is very important. The aim of this study was determine whether there is a significant correlation between the results of 1H-magnetic resonance spectroscopy (1H-MRS) and tumor invasive potential of astrocytoma, which is reflected by expression of matrix metalloproteinase-2 (MMP-2).

Methods

The 1H-MRS spectra of 41 histologically verified astrocytomas were obtained on a 3-T MR scanner. According to the World Health Organization classification criteria for central nervous system tumors, there were 16 low-grade astrocytomas (2 pilocytic astrocytomas, 14 grade II astrocytomas) and 25 high-grade astrocytomas (5 anaplastic astrocytomas, 20 glioblastomas).The choline/N-acetylaspartate (Cho/NAA) and choline/creatine (Cho/Cr) ratios were calculated. Of the 41 astrocytomas, 19 (8 low-grade and 11 high-grade) were analyzed immunohistochemically. Expression of MMP-2 was determined using streptavidin-peroxidase complex (SP) staining which was quantified by calculating its calibrated opacity density (COD) using an image analysis system. The correlations between metabolite ratios and the quantitative data from the immunohistochemical tests in the 19 astrocytomas were determined.

Results

The Cho/NAA and Cho/Cr ratios of high-grade astrocytoma were both significantly greater than those of low-grade astrocytoma (t = −6.222, P = 0.000; t = −6.533, P = 0.000, respectively). MMP-2 COD values of high-grade astrocytomas were also significantly greater than those of low-grade astrocytomas (t = −5.892, P = 0.000). There were strong positive correlations between Cho/NAA ratio and MMP-2 COD (r = 0.669, P = 0.002), and between Cho/Cr ratio and MMP-2 COD (r = 0.689, P = 0.001).

Conclusion

1H-MRS is helpful in evaluating the invasiveness of astrocytomas and predicting prognosis preoperatively by determining the Cho/NAA and Cho/Cr ratios.
Literature
1.
Rooprai HK, Rucklidge GJ, Panou C, Pilkington GJ (2000) The effects of exogenous growth factors on matrix metalloproteinase secretion by human brain tumour cells. Br J Cancer 82:52–55PubMedCrossRef
2.
Chintala SK, Tonn JC, Rao JS (1999) Matrix metalloproteinases and their biological function in human gliomas. Int J Dev Neurosci 17:495–502PubMedCrossRef
3.
Brinckerhoff CE, Matrisian LM (2002) Matrix metalloproteinases: a tail of a frog that became a prince. Nat Rev Mol Cell Biol 3:207–214PubMedCrossRef
4.
Yong VW, Power C, Forsyth P, Edwards DR (2001) Metalloproteinases in biology and pathology of the nervous system. Nat Rev Neurosci 2:502–511PubMedCrossRef
5.
Lampert K, Machein U, Machein MR, Conca W, Peter HH, Volk B (1998) Expression of matrix metalloproteinases and their tissue inhibitors in human brain tumors. Am J Pathol 153:429–437PubMed
6.
Shimizu H, Kumabe T, Shirane R, Yoshimoto T (2000) Correlation between choline level measured by proton MR spectroscopy and Ki-67 labeling index in gliomas. AJNR Am J Neuroradiol 21:659–665PubMed
7.
Gupta RK, Cloughesy TF, Sinha U, Garakian J, Lazareff J, Rubino G (2000) Relationships between choline magnetic resonance spectroscopy, apparent diffusion coefficient and quantitative histopathology in human glioma. J Neurooncol 50:215–226PubMedCrossRef
8.
Chen J, Huang SL, Li T, Chen XL (2006) In vivo research in astrocytoma cell proliferation with 1H-magnetic resonance spectroscopy: correlation with histopathology and immunohistochemistry. Neuroradiology 48:312–318PubMedCrossRef
9.
Stadlbauer A, Gruber S, Nimsky C, Fahlbusch R, Hammen T, Buslei R, Tomandl B, Moser E, Ganslandt O (2006) Preoperative grading of gliomas by using metabolite quantification with high-spatial-resolution proton MR spectroscopic imaging. Radiology 238:958–969PubMedCrossRef
10.
Möller-Hartmann W, Herminghaus S, Krings T, Marquardt G, Lanfermann H, Pilatus U, Zanella FE (2002) Clinical application of proton magnetic resonance spectroscopy in the diagnosis of intracranial mass lesions. Neuroradiology 44:371–381PubMedCrossRef
11.
Fountas KN, Kapsalaki EZ, Vogel RL, Fezoulidis I, Robinson JS, Gotsis ED (2004) Noninvasive histologic grading of solid astrocytomas using proton magnetic resonance spectroscopy. Stereotact Funct Neurosurg 82:90–97PubMedCrossRef
12.
Meyerand ME, Pipas JM, Mamourian A, Tosteson TD, Dunn JF (1999) Classification of biopsy-confirmed brain tumors using single-voxel MR spectroscopy. AJNR Am J Neuroradiol 20:117–123PubMed
13.
Dowling C, Bollen A, Noworolski SM, McDermott MW, Barbaro NM, Day MR (2001) Preoperative proton MR spectroscopy imaging of brain tumors: correlation with histopathologic analysis of resection specimens. AJNR Am J Neuroradiol 22:604–612PubMed
14.
Li X, Jin H, Lu Y, Oh J, Chang S, Nelson SJ (2004) Identification of MRI and 1H MRSI parameters that may predict survival for patients with malignant gliomas. NMR Biomed 17:10–20PubMedCrossRef
15.
Wu WC, Chen CY, Chung HW, Juan CJ, Hsueh CJ, Gao HW (2002) Discrepant MR spectroscopic and perfusion imaging results in a case of malignant transformation of cerebral glioma. AJNR Am J Neuroradiol 23:1775–1778PubMed
16.
Hu B, Guo P, Fang Q, Tao HQ, Wang D, Nagane M, Huang HJ, Gunji Y, Nishikawa R, Alitalo K, Cavenee WK, Cheng SY (2003) Angiopoietin-2 induces human glioma invasion through the activation of matrix metalloprotease-2. Proc Natl Acad Sci U S A 100:8904–8909PubMedCrossRef
17.
Takahashi M, Fukami S, Iwata N, Inoue K, Itohara S, Itoh H (2002) In vivo, glioma growth requires host derived matrix metalloproteinase 2 for maintenance of angioarchitecture. Pharmacol Res 46:155–163PubMedCrossRef
18.
Nafe R, Herminghaus S, Raab P, Wagner S, Pilatus U, Schneider B (2003) Preoperative proton-MR spectroscopy of gliomas – correlation with quantitative nuclear morphology in surgical specimen. J Neurooncol 63:233–245PubMedCrossRef
Metadata
Title
Evaluation of invasiveness of astrocytoma using 1H-magnetic resonance spectroscopy: correlation with expression of matrix metalloproteinase-2
Authors
Kai Zhang
Chuanfu Li
Ying Liu
Li Li
Xiangxing Ma
Xiangshui Meng
Dechao Feng
Publication date
01-11-2007
Publisher
Springer-Verlag
Published in
Neuroradiology / Issue 11/2007
Print ISSN: 0028-3940
Electronic ISSN: 1432-1920
DOI
https://doi.org/10.1007/s00234-007-0271-8

Other articles of this Issue 11/2007

Neuroradiology 11/2007 Go to the issue

Diagnostic Neuroradiology

Imaging of corticobasal degeneration syndrome

Interventional Neuroradiology

Carotid angioplasty and stenting in the elderly

Invited Review

Quantitative morphologic evaluation of magnetic resonance imaging during and after treatment of childhood leukemia

Functional Neuroradiology

Variability of clinical CT perfusion measurements in patients with carotid stenosis

Interventional Neuroradiology

Comparison of platinum and first-generation Matrix coils in under-packed canine side-wall aneurysms: evaluation of progressive thrombosis

Diagnostic Neuroradiology

MRI at 3 Tesla detects no evidence for ischemic brain damage in intensively treated patients with homozygous familial hypercholesterolemia