Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Osteoporosis International
Published in: Osteoporosis International 8/2017

01-08-2017 | Original Article

Comparison of total, free and bioavailable 25-OH vitamin D determinations to evaluate its biological activity in healthy adults: the LabOscat study

Authors: P. Peris, X. Filella, A. Monegal, N. Guañabens, L. Foj, M. Bonet, D. Boquet, E. Casado, D. Cerdá, A. Erra, C. Gómez-Vaquero, S. Martínez, N. Montalá, C. Pittarch, E. Kanterewicz, M. Sala, X. Suris, J. L. Carrasco, LabOscat Study Group

Published in: Osteoporosis International | Issue 8/2017

Login to get access

Abstract

Summary

Determination of different forms of 25-OHD (total, free and bioavailable) in healthy young women does not offer additional advantages over standard 25-OHDT for evaluating vitamin D deficiency. In these subjects 25-OHDT values <15 ng/ml would be more appropriate for defining this deficiency.

Introduction

Determination of 25-OH vitamin D serum levels (25-OHD) constitutes the method of choice for evaluating vitamin D deficiency. However, vitamin D-binding protein (DBP) may modulate its bioavailability thereby affecting correct evaluation of 25-OHD status. We analysed the impact of the determination of 25-OHD (total, free and bioavailable) on the evaluation its biologic activity (estimated by serum PTH determination) in healthy young women.

Methods

173 premenopausal women (aged 35–45 yrs.) were included. We analysed serum values of total 25-OHD (25-OHDT), DBP, albumin, PTH and bone formation (PINP,OC) and resorption (NTx,CTx) markers. Free(25-OHDF) and bioavailable (25-OHDB) serum 25-OHD levels were estimated by DBP and albumin determinations and also directly by ELISA (25-OHDF-2). We analysed threshold PTH values for the different forms of 25-OHD and the correlations and differences according to 25-OHDT levels <20 ng/ml.

Results

62% of subjects had 25-OHD values <20 ng/ml and also had significantly lower 25-OHDF and 25-OHDB values, with no significant differences in bone markers and PTH values. The PTH threshold value was similar for all forms of 25-OHD (∼70 pg/ml). Women with PTH values >70 had lower 25-OHDT (15.4 ± 1.4 vs. 18.3 ± 2.7, p < 0.05) and 25OHDB values (1.7 ± 0.2 vs. 2.2 ± 0.09, p < 0.05). The different forms of 25OHD were significantly intercorrelated, with marginal correlations between PTH and 25-OHDT (r = −0.136, p = 0.082).

Conclusions

Determination of different forms of 25-OHD in healthy young women does not offer additional advantages over standard 25-OHDT for evaluating vitamin D deficiency. In these subjects 25-OHDT values <15 ng/ml would be more appropriate for defining this deficiency.
Literature
1.
Lips P, Hosking D, Lippuner K, Norquist JM, Wehren L, Maalouf G et al (2006) The prevalence of vitamin D inadequacy amongst women with osteoporosis: an international epidemiological investigation. J Intern Med 260:245–254CrossRefPubMed
2.
3.
Fuleihan G-H, Bouillon R, Clarke B, Chakhtoura M, Cooper C, McClung M et al (2015) Serum 25-Hydroxyvitamin D levels: variability, knowledge gaps, and the concept of a desirable range. J Bone Miner Res 30:1119–1133CrossRef
4.
Holick MF, Siris ES, Binkley N, Beard MK, Khan A, Katzer JT et al (2005) Prevalence of vitamin D inadequacy among postmenopausal north American women receiving osteoporosis therapy. J Clin Endocrinol Metab 90:3215–3224CrossRefPubMed
5.
Harris SS, Soteriades E, Dawson-Hughes B (2001) Secondary hyperparathyroidism and bone turnover in elderly blacks and whites. J Clin Endocrinol Metab 86:3801–3804CrossRefPubMed
6.
Powe CE, Ricciardi C, Berg AH, Erdenesanaa D, Collerone G, Ankers E et al (2011) Vitamin D-binding protein modifies the vitamin D-bone mineral density relationship. J Bone Miner Res 26:1609–1616CrossRefPubMedPubMedCentral
7.
Powe CE, Evans MK, Wenger J, Zonderman AB, Berg AH, Nalls M et al (2013) Vitamin D-binding protein and vitamin D status of black Americans and white Americans. N Engl J Med 369:1991–2000CrossRefPubMedPubMedCentral
8.
Bikle D, Bouillon R, Thadhani R, Schoenmakers I (2017) Vitamin D metabolites in captivity? Should we measure free or total 25(OH)D to assess vitamin D status?. J Steroid Biochem Mol Biol. (in press)
9.
Holick MF (2013) Bioavailability of vitamin D and its metabolites in black and white adults. N Engl J Med 369:2047–2048CrossRefPubMed
10.
Guañabens N, Filella X, Monegal A, Gómez-Vaquero C, Bonet M, Buquet D et al (2016) Reference intervals for bone turnover markers in Spanish premenopausal women. Clin Chem Lab Med 54:293–303CrossRefPubMed
11.
Dawson-Hughes BN, Heaney RP, Holick MF, Lips P, Meunier PJ, Vieth R (2005) Estimates of optimal vitamin D status. Osteoporos Int 16:713–716CrossRefPubMed
12.
13.
14.
Ponda MP, McGee D, Breslow JL (2014) Vitamin D-binding protein levels do not influence the effect of vitamin D repletion on serum PTH and calcium: data from a randomized, controlled trial. J Clin Endocrinol Metab 99:2494–2499CrossRefPubMedPubMedCentral
15.
Dastani Z, Berger C, Langsetmo L, Fu L, Wong BY, Malik S et al (2014) In healthy adults, biological activity of vitamin D, as assessed by serum PTH, is largely independent of DBP concentrations. J Bone Miner Res 29:494–499CrossRefPubMed
16.
Schwartz JB, Lai J, Lizaola B, Kane L, Markova S, Weyland P et al (2014) A comparison of measured and calculated free 25(OH) vitamin D levels in clinical populations. J Clin Endocrinol Metab 99:1631–1637CrossRefPubMedPubMedCentral
17.
Denburg MR, Hoofnagle AN, Sayed S, Gupta J, de Boer IH, Appel LJ et al (2016) Chronic renal insufficiency cohort study investigators. Comparison of two ELISA methods and mass spectrometry for measurement of vitamin D-binding protein: implications for the assessment of bioavailable vitamin D concentrations across genotypes. J Bone Miner Res 31:1128–1136CrossRefPubMedPubMedCentral
18.
Abrams SA, Griffin IJ, Hawthorne KM, Gunn SK, Gundberg CM, Carpenter TO (2005) Relationships among vitamin D levels, parathyroid hormone, and calcium absorption in young adolescents J Clin Endocrinol Metab 90:5576–5581
19.
Saadi HF, Nagelkerke N, Benedict S, Qazaq HS, Zilahi E, Mohamadiyeh MK et al (2006) Predictors and relationships of serum 25 hydroxyvitamin D concentration with bone turnover markers, bone mineral density, and vitamin D receptor genotype in Emirati women. Bone 39:1136–1143
20.
Larijani B, Hossein-Nezhad A, Feizabad E, Maghbooli Z, Adibi H, Ramezani M et al (2016) Vitamin D deficiency, bone turnover markers and causative factors among adolescents: a cross-sectional study. J Diabetes Metab Disord 15:46
21.
Li M, Lv F, Zhang Z, Deng W, Li Y, Deng Z et al (2016) Establishment of a normal reference value of parathyroid hormone in a large healthy Chinese population and evaluation of its relation to bone turnover and bone mineral density. Osteoporos Int 27:1907–1916CrossRefPubMed
22.
Sahota O, Mundey MK, San P, Godber IM, Lawson N, Hosking DJ (2004) The relationship between vitamin D and parathyroid hormone: calcium homeostasis, bone turnover, and bone mineral density in postmenopausal women with established osteoporosis. Bone 35:312–319CrossRefPubMed
23.
Sai AJ, Walters RW, Fang X, Gallagher JC (2011) Relationship between vitamin D, parathyroid hormone, and bone health. J Clin Endocrinol Metab 96:E436–E446CrossRefPubMed
24.
Valcour A, Blocki F, Hawkins DM, Rao SD (2012) Effects of age and serum 25-OH-vitamin D on serum parathyroid hormone levels. J Clin Endocrinol Metab 97:3989–3995CrossRefPubMed
25.
Chapuy MC, Menier PP (1997) Vitamin D insufficiency in adults and the elderly. In: Feldman D (ed) Vitamin D. Academic Press, San Diego, pp p679–p693
26.
27.
Lamberg-Allardt CJ, Outila TA, Kärkkainen MU, Rita HJ, Valsta LM (2001) Vitamin D deficiency and bone health in healthy adults in Finland: could this be a concern in other parts of Europe? J Bone Miner Res 16:2066–2073CrossRefPubMed
28.
LeBlanc ES, Zakher B, Daeges M, Pappas M, Chou R (2015) Screening for vitamin D deficiency: a systematic review for the U.S. preventive services task force. Ann Intern Med 162:109–122CrossRefPubMed
Metadata
Title
Comparison of total, free and bioavailable 25-OH vitamin D determinations to evaluate its biological activity in healthy adults: the LabOscat study
Authors
P. Peris
X. Filella
A. Monegal
N. Guañabens
L. Foj
M. Bonet
D. Boquet
E. Casado
D. Cerdá
A. Erra
C. Gómez-Vaquero
S. Martínez
N. Montalá
C. Pittarch
E. Kanterewicz
M. Sala
X. Suris
J. L. Carrasco
LabOscat Study Group
Publication date
01-08-2017
Publisher
Springer London
Published in
Osteoporosis International / Issue 8/2017
Print ISSN: 0937-941X
Electronic ISSN: 1433-2965
DOI
https://doi.org/10.1007/s00198-017-4062-8

Other articles of this Issue 8/2017

Osteoporosis International 8/2017 Go to the issue

Review

Vibrational spectroscopic techniques to assess bone quality

Original Article

Use of dipeptidyl peptidase-4 inhibitors and risk of bone fracture in patients with type 2 diabetes in Germany—A retrospective analysis of real-world data

Original Article

Assessing the feasibility of the Effectiveness of Discontinuing Bisphosphonates trial: a pilot study

Original Article

A multicenter retrospective study of the risk factors associated with medication-related osteonecrosis of the jaw after tooth extraction in patients receiving oral bisphosphonate therapy: can primary wound closure and a drug holiday really prevent MRONJ?

Original Article

Undisturbed local bone formation capacity in patients with atypical femoral fractures: a case series

Original Article

Are bilateral decubitus views necessary in assessing for vertebral compression fractures using DXA vertebral fracture assessment?