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International Urogynecology Journal
Published in: International Urogynecology Journal 8/2019

01-08-2019 | Respiratory Microbiota | Original Article

Bladder urinary oxygen tension is correlated with urinary microbiota composition

Authors: Megan B. Shannon, Roberto Limeira, Danielle Johansen, Xiang Gao, Huaiying Lin, Qunfeng Dong, Alan J. Wolfe, Elizabeth R. Mueller

Published in: International Urogynecology Journal | Issue 8/2019

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Abstract

Introduction and hypothesis

Presence of microbial communities (microbiota) in an organ system depends on environmental factors, such as oxygen availability. We describe a novel technique to measure bladder urine oxygen tension (BUOT) in ambulatory women and use that technique to compare BUOT values to female urinary microbiota and participant urinary signs and symptoms.

Methods

Ambulatory female urogynecology patients presenting for clinical care who were willing to undergo transurethral catheterization underwent BUOT determination with a non-invasive flow-through oxygen sensor. To detect urinary microbiota in the bladder, 16S rRNA gene sequencing was performed on catheterized urine. Multivariate statistical analyses were performed to examine potential correlations among BUOT, urinary microbiota compositions and clinical variables.

Results

Significant variation in BUOT existed between individuals (range: 0.47–51.5 mmHg; median: 23.1 ± 13.5). Microbiota compositions were associated with BUOT (p = 0.03). BUOT was significantly lower in urines that were nitrite negative on dipstick analysis (p = 0.0001) and in participants who answered yes to having urinary leakage on the validated Urinary Distress Inventory (p = 0.01).

Conclusions

BUOTs can be measured in ambulatory women. For urogynecology patients, a wide range of values exist. BUOT may be associated with the presence of urinary microbiota and resultant signs and symptoms.
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Metadata
Title
Bladder urinary oxygen tension is correlated with urinary microbiota composition
Authors
Megan B. Shannon
Roberto Limeira
Danielle Johansen
Xiang Gao
Huaiying Lin
Qunfeng Dong
Alan J. Wolfe
Elizabeth R. Mueller
Publication date
01-08-2019
Publisher
Springer International Publishing
Published in
International Urogynecology Journal / Issue 8/2019
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI
https://doi.org/10.1007/s00192-019-03931-y

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