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01-06-2015 | Original Article

Myogenic stem cell-laden hydrogel scaffold in wound healing of the disrupted external anal sphincter

Authors: T. Ignacio Montoya, Jesus F. Acevedo, Benjamin Smith, Patrick W. Keller, Joseph L. Sailors, Liping Tang, R. Ann Word, Clifford Y. Wai

Published in: International Urogynecology Journal | Issue 6/2015

Abstract

Objective

To evaluate the effect of myogenic stem cell-laden hydrogel scaffold on contractile function and histomorphology of the external anal sphincter (EAS) after transection without repair.

Methods

Eighty female rats underwent anal sphincter transection without repair. After 2 weeks, animals were injected at the transection site with: nothing (non-repaired control, NRC group); a polyethylene glycol-based hydrogel matrix scaffold combined with phosphate-buffered saline (PBS/hydrogel group); a hydrogel matrix scaffold combined with myogenic stem cells (stem cell/hydrogel group): or type I collagen (collagen) group. 4 (n = 40) or 12 (n = 40) weeks later, the anal sphincter complexes were dissected out and analyzed for contractile function, disruption, and striated muscle volume. Time-matched unoperated controls (UOC) were utilized for each of the two time points (n = 20).

Results

After 4 weeks, maximal electrical field-stimulated (EFS) contractions were significantly decreased in all four non-repaired treatment groups compared with UOC. However, EFS-stimulated contractions, tetanic force generation, and twitch tension were improved in non-repaired EAS injected with stem cell/hydrogel group relative to the NRC, PBS/hydrogel, or collagen groups. NRC and sphincters injected with PBS/hydrogel deteriorated further by 12 weeks, while those receiving stem cell/hydrogel maintained improved contractile function at varying frequencies and voltages. Striated muscle volume increased from 4 to 12 weeks for PBS/hydrogel and stem cell/hydrogel animals. At 12 weeks, stem cell/hydrogel animals had greater sphincter striated muscle volumes compared with all other treatment groups.

Conclusion

In this animal model, sustained improvement of contractile responses in non-repaired EAS treated with biogel scaffold and myogenic stem cells suggests that a biologically compatible matrix may facilitate stem cell survival, differentiation, or function leading to recovery of contractile function even after persistent disruption.

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Title: Myogenic stem cell-laden hydrogel scaffold in wound healing of the disrupted external anal sphincter
Authors: T. Ignacio Montoya
Jesus F. Acevedo
Benjamin Smith
Patrick W. Keller
Joseph L. Sailors
Liping Tang
R. Ann Word
Clifford Y. Wai
Publication date: 01-06-2015
Publisher: Springer London
Published in: International Urogynecology Journal / Issue 6/2015
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-014-2620-6

At a glance: The STEP trials

Springer Medicine

Myogenic stem cell-laden hydrogel scaffold in wound healing of the disrupted external anal sphincter

Abstract

Objective

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Methods

Results

Conclusion

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