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Open Access 01-09-2013 | Original Article

Fibroblasts from women with pelvic organ prolapse show differential mechanoresponses depending on surface substrates

Authors: Alejandra M. Ruiz-Zapata, Manon H. Kerkhof, Behrouz Zandieh-Doulabi, Hans A. M. Brölmann, Theo H. Smit, Marco N. Helder

Published in: International Urogynecology Journal | Issue 9/2013

Abstract

Introduction and hypothesis

Little is known about dynamic cell-matrix interactions in the context of pathophysiology and treatments for pelvic organ prolapse (POP). This study sought to identify differences between fibroblasts from women with varying degrees of prolapse in reaction to mechanical stimuli and matrix substrates in vitro.

Methods

Fibroblasts from the vaginal wall of three patients with POP Quantification (POP-Q) system stages 0, II, and IV were stretched on artificial polymer substrates either coated or not coated with collagen I. Changes in morphology and anabolic/catabolic compounds that affect matrix remodelling were evaluated at protein- and gene-expression levels. Statistical analysis was performed using one-way analysis of variance (ANOVA), followed by Tukey-Kramer’s post hoc test.

Results

POP fibroblasts show delayed cell alignment and lower responses to extracellular matrix remodelling factors at both enzymatic- and gene-expression levels compared with healthy fibroblasts.

Conclusion

POP fibroblasts, when compared with healthy cells, show differential mechanoresponses on two artificial polymer substrates. This should be taken into account when designing or improving implants for treating POP.

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Title: Fibroblasts from women with pelvic organ prolapse show differential mechanoresponses depending on surface substrates
Authors: Alejandra M. Ruiz-Zapata
Manon H. Kerkhof
Behrouz Zandieh-Doulabi
Hans A. M. Brölmann
Theo H. Smit
Marco N. Helder
Publication date: 01-09-2013
Publisher: Springer London
Published in: International Urogynecology Journal / Issue 9/2013
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-013-2069-z

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Fibroblasts from women with pelvic organ prolapse show differential mechanoresponses depending on surface substrates

Abstract

Introduction and hypothesis

Methods

Results

Conclusion

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