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Published in: International Urogynecology Journal 9/2010

01-09-2010 | Original Article

Tissue-engineered fascia from vaginal fibroblasts for patientsneeding reconstructive pelvic surgery

Authors: Man-Jung Hung, Mei-Chin Wen, Chia-Nung Hung, Esther Shih-Chu Ho, Gin-Den Chen, Vivian Cheng Yang

Published in: International Urogynecology Journal | Issue 9/2010

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Abstract

Introduction and hypothesis

Mesh-augmented reconstructive surgery for pelvic organ prolapse (POP) does not meet clinical expectations. A tissue-engineered fascia equivalent needs to be developed.

Methods

Human vaginal fibroblasts (HVFs) from 10 patients were characterized in vitro. Eligible HVFs and a biodegradable scaffold were used to fabricate a fascia equivalent, which was then transplanted in vivo.

Results

The cultured HVFs were divided into high (n = 6) or low (n = 4) collagen I/III ratio groups. Cells of the high-ratio group exhibited significantly higher proliferation potential than those of the low-ratio group (P < 0.05). A fascia equivalent was made with HVFs of the high-ratio group. In the subsequent animal study, a well-organized neo-fascia formation containing HVFs could be traced up to 12 weeks after transplantation.

Conclusions

Our results suggest that a tissue-engineered fascia could be developed from HVFs in vitro and in vivo, which might be an effective treatment for POP in the future.
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Metadata
Title
Tissue-engineered fascia from vaginal fibroblasts for patientsneeding reconstructive pelvic surgery
Authors
Man-Jung Hung
Mei-Chin Wen
Chia-Nung Hung
Esther Shih-Chu Ho
Gin-Den Chen
Vivian Cheng Yang
Publication date
01-09-2010
Publisher
Springer-Verlag
Published in
International Urogynecology Journal / Issue 9/2010
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI
https://doi.org/10.1007/s00192-010-1168-3

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