Top

Published in:

01-08-2008 | Original Article

Impact of the structure of polypropylene meshes in local tissue reaction: in vivo stereological study

Authors: Cassio Riccetto, Ricardo Miyaoka, Rogerio de Fraga, Regina Barbosa, Miriam Dambros, Andreza Teixeira, Paulo Palma

Published in: International Urogynecology Journal | Issue 8/2008

Abstract

Polypropylene slings are monofilament and multifilament meshes. Their features can impact clinical outcomes. We analyzed tissue reaction, inflammation, and the density of collagen fibers elicited by polypropylene meshes in the subcutaneous tissue of female rats. Thirty female Wistar rats underwent the implantation of 8×4-mm polypropylene meshes (two monofilamentar and two multifilamentar). The rats were killed at 7, 60, and 120 days after implant. Stereology was used to analyze the collagen fiber density. Acute inflammation was more intense in the early post-implant period (p=0.006), except for intravaginal slingplasty (p=0.342). Lymphoplasmocytic reaction was more intense in multifilament samples, even after 60 days (p<0.001) and 120 days (p=0.001). The stereological analysis showed significant differences between mono- and multifilament meshes, considering that monofilament meshes induced more collagen deposition and a greater density of collagen fibers (p<0.001). Multifilament meshes produced a more granulomatous reaction. The collagen fiber density was greater in monofilament meshes, which induced more favorable biocompatibility assessed objectively.

Abrams P, Cardozo L, Fall M, Griffiths D, Rosier P, Ulmsten U, van Kerrebroeck P, Victor A, Wein A (2002) The standardisation of terminology of lower urinary tract function: report from the Standardisation Sub-committee of the International Continence Society. Neurourol Urodyn 21:167–178PubMedCrossRef

Jensen JK, Rufford HJ (2001) Sling procedures—artificial. In: Cardozo L, Staskin D (eds) Textbook of female urology and urogynecology, 1st edn. Isis Medical Media, London, pp 543–551

Amid PK (1997) Classification of biomaterials and their related complications in abdominal wall hernia surgery. Hernia 1:15–21CrossRef

Slack M, Sandhu JS, Staskin DR, Grant RC (2006) In vivo comparison of suburethral sling materials. Int Urogynecol J Pelvic Floor Dysfunct 17:106–110PubMedCrossRef

Baessler K, Hewson A, Tunn R, Quatawneh A, Maher C (2003) Intravaginal sling distress. Neurol Urodyn 22:408

Glavind K, Sander P (2004) Erosion, defective healing and extrusion after tension-free urethropexy for the treatment of stress urinary incontinence. Int Urogynecol J Pelvic Floor Dysfunct 15:179–182PubMedCrossRef

Siegel AL, Kim M, Goldstein M, Levey S, Ilbeigi P (2005) High incidence of vaginal mesh extrusion using the intravaginal slingplasty sling. J Urol 174(4 Pt 1):1308–1311PubMedCrossRef

Bafghi A, Benizri EI, Trastour C, Benizri EJ, Michiels JF, Bongain A (2005) Multifilament polypropylene mesh for urinary incontinence: 10 cases of infections requiring removal of the sling. BJOG 112:376–378PubMedCrossRef

Reisenauer C, Wallwiener D, Stenzl A, Solomayer FE, Sievert KD (2006) Urethrovaginal fistula—a rare complication after the placement of a suburethral sling (IVS). Int Urogynecol J Pelvic Floor Dysfunct 18:343–346PubMed

10.

Weibel ER, Kistler GS, Scherle WF (1966) Practical stereological methods for morphometric cytology. J Cell Biol 30:23–38PubMedCrossRef

11.

Mandarim-de-Lacerda CA (1999) What is the interest of normal and pathological morphological research to be quantitative? The example of stereology. Braz J Morphol Sci 16:131–139

12.

Thiel M, Rodrigues Palma PC, Riccetto CLZ, Dambros M, Netto NR Jr (2005) A stereological analysis of fibrosis and inflammatory reaction induced by four different synthetic slings. BJU Int 95:833–837PubMedCrossRef

13.

Hanson D, Langemo D, Thompson P, Anderson J, Hunter S (2005) Understanding wound fluid and the phases of healing. Adv Skin Wound Care 18(7):360–362PubMedCrossRef

14.

Bobyn JD, Wilson GJ, MacGregor DC, Pilliar RM, Weatherly GC (1982) Effect of pore size on the peel strength of attachment of fibrous tissue to porous-surfaced implants. J Biomed Mater Res 16:571–584PubMedCrossRef

15.

Brun JL, Bordenave L, Lefebvre F, Bareille R, Barbie C, Rouais F, Baquey CH (1992) Physical and biological characteristics of the main biomaterials used in pelvic surgery. Biomed Mater Eng 2:203–225PubMed

16.

Homsy CA, Kent JN, Hinds EC (1973) Materials for oral implantation—biological and functional criteria. J Am Dental Assoc 86:817–832

17.

Krause HG, Goh JTW, Khoo SK, Williams R, Galloway SJ (2004) Biocompatible properties of surgical mesh using an animal model. Neurol Urodyn 23:Abstract 20

Title: Impact of the structure of polypropylene meshes in local tissue reaction: in vivo stereological study
Authors: Cassio Riccetto
Ricardo Miyaoka
Rogerio de Fraga
Regina Barbosa
Miriam Dambros
Andreza Teixeira
Paulo Palma
Publication date: 01-08-2008
Publisher: Springer-Verlag
Published in: International Urogynecology Journal / Issue 8/2008
Print ISSN: 0937-3462
Electronic ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-008-0582-2

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Impact of the structure of polypropylene meshes in local tissue reaction: in vivo stereological study

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 8/2008

Family history as a risk factor for pelvic organ prolapse

A randomized trial comparing TVT with TVT-O: 12-month results

Clinical and ultrasonographic correlations following three surgical anti-incontinence procedures (TOT, TVT and TVT-O)

Arcus to arcus microsling: technique and preliminary results

A microbiological and immunohistochemical analysis of periurethral and vaginal tissue in women with de novo urge symptoms after mid-urethral sling procedures—a prospective case-controlled study

Validity and reproducibility of the digital detection of levator trauma