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Knee Surgery, Sports Traumatology, Arthroscopy

Published in:

01-08-2013 | Experimental Study

Anti-inflammatory cytokine profile in early human tendon repair

Authors: P. W. Ackermann, E. Domeij-Arverud, P. Leclerc, P. Amoudrouz, G. A. Nader

Published in: Knee Surgery, Sports Traumatology, Arthroscopy | Issue 8/2013

Abstract

Purpose

The aim of this study was to assess inflammation and the presence and relative levels of cytokines, which may be involved in regulating early human Achilles tendon healing.

Methods

Nine patients with acute Achilles tendon rupture were included, operated on and post-operatively immobilized. Two weeks post-operatively, microdialysis of the peritendinous interstitial compartment was performed in the healing and intact contralateral Achilles tendons. Quantification of tumour necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70 and IL-17A was accomplished using a cytometric bead array. Prostaglandin (PG) E₂ levels were measured by enzyme immunoassay.

Results

None of the patients displayed detectable PGE₂ levels. Pro-inflammatory cytokines were below detection levels (IFNγ, IL-12, and IL-17) or did not differ between injured and control tendons (IL-1β and TNF). Notably, IL-6, IL-8 and IL-10 concentrations in the healing Achilles tendon were significantly elevated: 13-fold (p = 0.009), 28-fold (p = 0.02), and 3.7-fold (p = 0.03), respectively.

Conclusion

At 2 weeks post–human Achilles tendon rupture, healing is characterized by a resolving inflammatory phase and up-regulation of IL-6, IL-8 and IL-10. The absence of inflammation suggests that at this time point, these cytokines may be associated with anti-inflammatory and regenerative effects on the tendon healing process.

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Title: Anti-inflammatory cytokine profile in early human tendon repair
Authors: P. W. Ackermann
E. Domeij-Arverud
P. Leclerc
P. Amoudrouz
G. A. Nader
Publication date: 01-08-2013
Publisher: Springer Berlin Heidelberg
Published in: Knee Surgery, Sports Traumatology, Arthroscopy / Issue 8/2013
Print ISSN: 0942-2056
Electronic ISSN: 1433-7347
DOI: https://doi.org/10.1007/s00167-012-2197-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Anti-inflammatory cytokine profile in early human tendon repair

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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