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Intensive Care Medicine
Published in: Intensive Care Medicine 12/2011

01-12-2011 | Experimental

A metabolomic approach for diagnosis of experimental sepsis

Authors: José L. Izquierdo-García, Nicolás Nin, Jesús Ruíz-Cabello, Yeny Rojas, Marta de Paula, Sonia López-Cuenca, Luis Morales, Leticia Martínez-Caro, Pilar Fernández-Segoviano, Andrés Esteban, José A. Lorente

Published in: Intensive Care Medicine | Issue 12/2011

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Abstract

Background

The search for reliable diagnostic biomarkers of sepsis remains necessary. Assessment of global metabolic profiling using quantitative nuclear magnetic resonance (NMR)-based metabolomics offers an attractive modern methodology for fast and comprehensive determination of multiple circulating metabolites and for defining the metabolic phenotype of sepsis.

Objective

To develop a novel NMR-based metabolomic approach for diagnostic evaluation of sepsis.

Methods

Male Sprague–Dawley rats (weight 325–375 g) underwent cecal ligation and puncture (n = 14, septic group) or sham procedure (n = 14, control group) and 24 h later were euthanized. Lung tissue, bronchoalveolar lavage (BAL) fluid, and serum samples were obtained for 1H NMR and high-resolution magic-angle spinning analysis. Unsupervised principal components analysis was performed on the processed spectra, and a predictive model for diagnosis of sepsis was constructed using partial least-squares discriminant analysis.

Results

NMR-based metabolic profiling discriminated characteristics between control and septic rats. Characteristic metabolites changed markedly in septic rats as compared with control rats: alanine, creatine, phosphoethanolamine, and myoinositol concentrations increased in lung tissue; creatine increased and myoinositol decreased in BAL fluid; and alanine, creatine, phosphoethanolamine, and acetoacetate increased whereas formate decreased in serum. A predictive model for diagnosis of sepsis using these metabolites classified cases with sensitivity and specificity of 100%.

Conclusions

NMR metabolomic analysis is a potentially useful technique for diagnosis of sepsis. The concentrations of metabolites involved in energy metabolism and in the inflammatory response change in this model of sepsis.
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Metadata
Title
A metabolomic approach for diagnosis of experimental sepsis
Authors
José L. Izquierdo-García
Nicolás Nin
Jesús Ruíz-Cabello
Yeny Rojas
Marta de Paula
Sonia López-Cuenca
Luis Morales
Leticia Martínez-Caro
Pilar Fernández-Segoviano
Andrés Esteban
José A. Lorente
Publication date
01-12-2011
Publisher
Springer-Verlag
Published in
Intensive Care Medicine / Issue 12/2011
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI
https://doi.org/10.1007/s00134-011-2359-1

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