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Intensive Care Medicine

Published in:

01-04-2004 | Original

Neutrophil chemotaxis and receptor expression in clinical septic shock

Authors: Ahmad D. Chishti, Brian K. Shenton, John A. Kirby, Simon V. Baudouin

Published in: Intensive Care Medicine | Issue 4/2004

Abstract

Objective

To examine the hypothesis that neutrophil chemotaxis to interleukin-8 (IL-8) is reduced in septic shock. Surface expression of neutrophil CXC chemokine receptors and the adhesion molecule CD11b were also examined and associations between disease severity, gas exchange and receptor expression were studied.

Design

Prospective cohort clinical study.

Setting

Intensive care unit in a tertiary referral teaching hospital.

Patients

Patients with septic shock (n=15) and healthy controls (n=8) were studied.

Measurements and results

Daily (for 5 consecutive days) flow cytometric measurements of chemokine and β integrin surface expression. “In vitro” neutrophil chemotaxis to IL-8 was also compared between patients with sepsis and healthy controls. CXCR2 expression significantly fell, CD11b expression increased and CXCR1 expression was unchanged throughout the study in the septic group compared with healthy controls. CD11b positively correlated with increasing APACHE II scores (p<0.0001) and worsening PaO₂/FIO₂ ratios (p<0.0001). CXCR2 expression negatively correlated with both APACHE II scores (p=0.016) and PaO₂/FIO₂ ratios (p=0.01). There was no correlation between CXCR1 expression and either APACHE II score or PaO₂/FIO₂ ratios. Chemotaxis to IL-8 was reduced in patients with sepsis compared with healthy volunteers.

Conclusions

Surface expression of the chemokine receptor CXCR2 and the β-integrin CD11b, but not CXCR1, were reduced on neutrophils isolated from patients with septic shock compared with healthy controls. Chemotaxis to IL-8 was also reduced in neutrophils from septic patients compared with healthy controls. The changes in receptor expression correlated with measures of disease severity.

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Title: Neutrophil chemotaxis and receptor expression in clinical septic shock
Authors: Ahmad D. Chishti
Brian K. Shenton
John A. Kirby
Simon V. Baudouin
Publication date: 01-04-2004
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 4/2004
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-004-2175-y

At a glance: The STEP trials

Springer Medicine

Neutrophil chemotaxis and receptor expression in clinical septic shock

Abstract

Objective

Design

Setting

Patients

Measurements and results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Design

Setting

Patients

Measurements and results

Conclusions

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