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Intensive Care Medicine

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01-03-2004 | Experimental

Expression of nitric oxide synthase, aquaporin 1 and aquaporin 5 in rat after bleomycin inhalation

Authors: An-Soo Jang, Jong-Un Lee, Inseon-S Choi, Kyung-Ok Park, June Hyuk Lee, Sung-Woo Park, Choon-Sik Park

Published in: Intensive Care Medicine | Issue 3/2004

Abstract

Objective

Nitric oxide (NO) and aquaporins (AQPs) are believed to play an important role in the pathogenesis of pulmonary inflammation and edema. The aim of this study was to investigate the role of NO synthase (NOS) and AQP in acute lung injury (ALI) lung following bleomycin inhalation in rats.

Design and setting

A prospective controlled trial in a university research laboratory.

Animals and interventions

Sprague-Dawley rats were treated by inhalation of 10 U/kg bleomycin hydrochloride in 5 ml of normal saline. Control rats were treated with 5 ml normal saline alone. The animals (6–8 rats per group) were killed on days 4, 7 or 14.

Measurements and results

We analyzed the change in expression of inducible NOS (iNOS), neuronal NOS (nNOS), endothelial NOS (eNOS), aquaporin 1 (AQP1) and aquaporin 5 (AQP5) over time by Western blot. Nitrate and nitrite concentrations were measured in bronchoalveolar lavage fluid (BALF) using a modified Griess reaction. The nitrite and nitrate concentrations in BALF from rats 4 days after bleomycin exposure were greater than those from saline-treated rats. Immunoblotting studies demonstrated increased levels of eNOS in the rat lung at 4, 7 and 14 days and iNOS at 7 and 14 days after bleomycin inhalation. However, nNOS expression was unaltered. Although AQP1 expression was decreased in rats at 4 days, AQP5 expression was increased at 4, 7 and 14 days.

Conclusions

This study demonstrates that NO metabolites increase along with eNOS and iNOS expression during the acute exudative phase in ALI, and that AQP and NOS are regulated independently in bleomycin-induced pulmonary edema.

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Title: Expression of nitric oxide synthase, aquaporin 1 and aquaporin 5 in rat after bleomycin inhalation
Authors: An-Soo Jang
Jong-Un Lee
Inseon-S Choi
Kyung-Ok Park
June Hyuk Lee
Sung-Woo Park
Choon-Sik Park
Publication date: 01-03-2004
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 3/2004
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-003-2129-9

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Expression of nitric oxide synthase, aquaporin 1 and aquaporin 5 in rat after bleomycin inhalation

Abstract

Objective

Design and setting

Animals and interventions

Measurements and results

Conclusions

At a glance: The ONWARDS insulin icodec trials

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Abstract

Objective

Design and setting

Animals and interventions

Measurements and results

Conclusions

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