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01-02-2004 | Experimental

Effects of C1 esterase inhibitor administration on intestinal functional capillary density, leukocyte adherence and mesenteric plasma extravasation during experimental endotoxemia

Authors: Christian Lehmann, Jürgen Birnbaum, Carsten Lührs, Oskar Rückbeil, Claudia Spies, Sabine Ziemer, Matthias Gründling, Dragan Pavlovic, Taras Usichenko, Michael Wendt, Wolfgang J. Kox

Published in: Intensive Care Medicine | Issue 2/2004

Abstract

Objective

To determine the effects of C1 esterase inhibitor (C1-INH) administration on intestinal functional capillary density, leukocyte adherence, and mesenteric plasma extravasation during experimental endotoxemia.

Design and setting

Prospective, randomized, controlled animal study in the experimental laboratory of a university.

Subjects

42 male Wistar rats.

Interventions

The animals were divided into three groups. One half of the animals of each group underwent studies of intestinal functional capillary density and leukocyte adherence on venular endothelium by intravital fluorescence microscopy. In the other half of the animals mesenteric plasma extravasation (FITC albumin) was determined by intravital fluorescence microscopy. Treatment groups received endotoxin infusion of 2.5 mg/kg per hour (group 2 and 3) and 100 U/kg b.w. C1-INH (group 3) during the 2 h of endotoxemia.

Measurements and results

Endotoxemia resulted in a significant decrease in mucosal functional capillary density (18.5% vs. controls), which was reduced by C1-INH administration (9.5%). Treatment with C1-INH also significantly attenuated intestinal leukocyte adherence in submucosal venules (35% vs. endotoxin group) and mesenteric plasma extravasation (44% vs. endotoxin group).

Conclusions

C1-INH administration diminishes endotoxin-induced changes in the intestinal microcirculation during experimental endotoxemia.

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Title: Effects of C1 esterase inhibitor administration on intestinal functional capillary density, leukocyte adherence and mesenteric plasma extravasation during experimental endotoxemia
Authors: Christian Lehmann
Jürgen Birnbaum
Carsten Lührs
Oskar Rückbeil
Claudia Spies
Sabine Ziemer
Matthias Gründling
Dragan Pavlovic
Taras Usichenko
Michael Wendt
Wolfgang J. Kox
Publication date: 01-02-2004
Publisher: Springer Berlin Heidelberg
Published in: Intensive Care Medicine / Issue 2/2004
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-003-2042-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of C1 esterase inhibitor administration on intestinal functional capillary density, leukocyte adherence and mesenteric plasma extravasation during experimental endotoxemia

Abstract

Objective

Design and setting

Subjects

Interventions

Measurements and results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Design and setting

Subjects

Interventions

Measurements and results

Conclusions

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