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01-10-2003 | Experimental

Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation

Authors: Cristiane Ritter, Michael Andrades, Mário Luis C. Frota Jr., Fernanda Bonatto, Ricardo A. Pinho, Manuela Polydoro, Fábio Klamt, Cleovaldo T. S. Pinheiro, Sérgio S. Menna-Barreto, José Cláudio F. Moreira, Felipe Dal-Pizzol

Published in: Intensive Care Medicine | Issue 10/2003

Abstract

Objective

This study assessed parameters of free radical damage to biomolecules, mitochondrial superoxide production, superoxide dismutase, and catalase activities and their relationship to sepsis mortality.

Design and setting

Prospective animal study in a university laboratory for experimental.

Subjects

140 male Wistar rats.

Interventions

The animals were randomly divided into three groups: sham-operated (n=20), cecal ligation and perforation resuscitated with normal saline (n=40), and cecal ligation and perforation with normal saline plus antibiotics (n=40).

Measurements and results

Blood samples were collected from all animals 3, 12, and 24 h after CLP through a jugular catheter inserted before CLP. Rats were evaluated during 5 days after the intervention. Nonsurvivor animals were grouped according to the duration between sepsis induction and death, and oxidative parameters were compared to survivors and sham-operated. Lipid peroxidation, protein carbonyls, and superoxide dismutase were significantly increased in nonsurvivor septic rats and were predictive of mortality. We demonstrated that there is a different modulation of superoxide dismutase and catalase in nonsurvivors during the course of septic response. There was a marked increase in superoxide dismutase activity without a proportional increase in catalase activity in nonsurvivors.

Conclusions

This is the first report of plasma superoxide dismutase as an earlier marker of mortality. Ours results might help to clarify an important aspect of oxidative response to sepsis, i.e., an increase in superoxide dismutase activity without a proportional increase in catalase activity

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Title: Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation
Authors: Cristiane Ritter
Michael Andrades
Mário Luis C. Frota Jr.
Fernanda Bonatto
Ricardo A. Pinho
Manuela Polydoro
Fábio Klamt
Cleovaldo T. S. Pinheiro
Sérgio S. Menna-Barreto
José Cláudio F. Moreira
Felipe Dal-Pizzol
Publication date: 01-10-2003
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 10/2003
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-003-1789-9

At a glance: The STEP trials

Springer Medicine

Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation

Abstract

Objective

Design and setting

Subjects

Interventions

Measurements and results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Design and setting

Subjects

Interventions

Measurements and results

Conclusions

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