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European Journal of Trauma and Emergency Surgery
Published in: European Journal of Trauma and Emergency Surgery 1/2022

Open Access 01-02-2022 | Bone Defect | Original Article

Impact of scaffold granule size use in Masquelet technique on periosteal reaction: a study in rat femur critical size bone defect model

Authors: Maximilian Leiblein, Andreas Winkenbach, Elias Koch, Alexander Schaible, Hubert Büchner, Ingo Marzi, Dirk Henrich, Christoph Nau

Published in: European Journal of Trauma and Emergency Surgery | Issue 1/2022

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Abstract

Purpose

The Masquelet technique for the treatment of large bone defects is a two-stage procedure based on an induced membrane. Compared to mature periosteum, the induced membrane differs significantly. However, both play a crucial role in bone regeneration. As part of a histological and radiological post-evaluation of an earlier project, we analyzed the influence of the granule size of the bone void filler Herafill® on development of periosteum regrowth in a critical size defect.

Methods

We compared three different sizes of Herafill® granules (Heraeus Medical GmbH, Wehrheim) in vivo in a rat femoral critical size defect (10 mm) treated with the induced membrane technique. After 8 weeks healing time, femurs were harvested and taken for histological and radiological analysis.

Results

A significantly increased regrowth of periosteum into the defect was found when small granules were used. Large granules showed significantly increased occurrence of bone capping. Small granules lead to significant increase in callus formation in the vicinity to the membrane.

Conclusion

The size of Herafill® granules has significant impact on the development of periosteal-like structures around the defect using Masquelet’s induced membrane technique. Small granules show significantly increased regrowth of periosteum and improved bone formation adjacent to the induced membrane.
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Metadata
Title
Impact of scaffold granule size use in Masquelet technique on periosteal reaction: a study in rat femur critical size bone defect model
Authors
Maximilian Leiblein
Andreas Winkenbach
Elias Koch
Alexander Schaible
Hubert Büchner
Ingo Marzi
Dirk Henrich
Christoph Nau
Publication date
01-02-2022
Publisher
Springer Berlin Heidelberg
Keyword
Bone Defect
Published in
European Journal of Trauma and Emergency Surgery / Issue 1/2022
Print ISSN: 1863-9933
Electronic ISSN: 1863-9941
DOI
https://doi.org/10.1007/s00068-020-01516-9

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