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Strahlentherapie und Onkologie
Published in: Strahlentherapie und Onkologie 6/2015

01-06-2015 | Original Article

Expression of transforming growth factor beta 1-related signaling proteins in irradiated vessels

Authors: Raimund H. M. Preidl, M.D., Patrick Möbius, D.M.D., Manuel Weber, M.D., Kerstin Amann, M.D., Ph.D., Friedrich W. Neukam, M.D., D.M.D., Ph.D., Andreas Schlegel, M.D., D.M.D., Falk Wehrhan, M.D., D.M.D.

Published in: Strahlentherapie und Onkologie | Issue 6/2015

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Abstract

Aim

Microvascular free tissue transfer is a standard method in head and neck reconstructive surgery. However, previous radiotherapy of the operative region is associated with an increased incidence in postoperative flap-related complications and complete flap loss. As transforming growth factor beta (TGF-β) 1 and galectin-3 are well known markers in the context of fibrosis and lectin-like oxidized low-density lipoprotein 1 (LOX-1) supports vascular atherosclerosis, the aim of this study was to evaluate the expression of TGF-β1 and related markers as well as LOX-1 in irradiated vessels.

Materials and methods

To evaluate the expression of galectin-3, Smad 2/3, TGF-β1, and LOX-1, 20 irradiated and 20 nonirradiated arterial vessels were used for immunohistochemical staining. We semiquantitatively assessed the ratio of stained cells/total number of cells (labeling index).

Results

Expression of galectin-3, Smad 2/3, and TGF-β1 was significantly increased in previously irradiated vessels compared with nonirradiated controls. Furthermore, LOX-1 was expressed significantly higher in irradiated compared with nonirradiated vessels.

Conclusion

Fibrosis-related proteins like galectin-3, Smad 2/3, and TGF-β1 are upregulated after radiotherapy and support histopathological changes leading to vasculopathy of the irradiated vessels. Furthermore, postoperative complications in irradiated patients can be explained by increased endothelial dysfunction caused by LOX-1 in previously irradiated patients. Consequently, not only TGF-β1 but also galectin-3inhibitors may decrease complications after microsurgical tissue transfer.
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Metadata
Title
Expression of transforming growth factor beta 1-related signaling proteins in irradiated vessels
Authors
Raimund H. M. Preidl, M.D.
Patrick Möbius, D.M.D.
Manuel Weber, M.D.
Kerstin Amann, M.D., Ph.D.
Friedrich W. Neukam, M.D., D.M.D., Ph.D.
Andreas Schlegel, M.D., D.M.D.
Falk Wehrhan, M.D., D.M.D.
Publication date
01-06-2015
Publisher
Springer Berlin Heidelberg
Published in
Strahlentherapie und Onkologie / Issue 6/2015
Print ISSN: 0179-7158
Electronic ISSN: 1439-099X
DOI
https://doi.org/10.1007/s00066-014-0797-8

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