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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

Open Access 26-02-2024 | Laser | Original Article

Biofilm volume and acidification within initial biofilms formed in situ on buccally and palatally exposed bracket material

Authors: Micha Frederic Loewe, Katharina Doll-Nikutta, Meike Stiesch, Rainer Schwestka-Polly

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

Abstract

Purpose

Acidification by bacterial biofilms at the bracket/tooth interface is one of the most common problems in fixed orthodontic treatments, which can lead to white spot lesions (WSL) and caries. As lingual brackets were shown to exhibit reduced WSL formation clinically, the aim of this in situ study was to compare initial intraoral biofilm formation and acidification on bracket-like specimens placed buccally and palatally in the upper jaw as a possible cause for this observation.

Methods

Intraoral biofilm was collected from splints equipped with buccally and palatally exposed test specimens, which were worn by 12 volunteers for a total of 48 h. The test specimens consisted of standard bracket material cylinders on top of a hydroxyapatite disc to represent the bracket/tooth interface. They were analyzed for three-dimensional biofilm volume and live/dead distribution by fluorescence staining and confocal laser scanning microscopy as well as for acidification by fluorescence-based pH ratiometry.

Results

Similar general biofilm morphology with regard to volume and viability could be detected for buccally and palatally exposed specimens. For pH values, biofilms from both positions showed increased acidification at the bottom layer. Interestingly, the pH value at the top layers of the biofilms was slightly lower on palatally than on buccally exposed specimens, which may likely be due to anatomic conditions.

Conclusion

Based on the results of this study, initial intraoral biofilm formation and acidification is almost similar on the bracket material/biomimetic tooth interface when placed buccally or palatally in the upper jaw. As lingual brackets were shown to exhibit reduced WSL formation clinically, future studies should investigate further factors like bracket geometry.

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Title: Biofilm volume and acidification within initial biofilms formed in situ on buccally and palatally exposed bracket material
Authors: Micha Frederic Loewe
Katharina Doll-Nikutta
Meike Stiesch
Rainer Schwestka-Polly
Publication date: 26-02-2024
Publisher: Springer Medizin
Keyword: Laser
Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-024-00515-4

At a glance: The STEP trials

Springer Medicine

Biofilm volume and acidification within initial biofilms formed in situ on buccally and palatally exposed bracket material

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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