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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

05-01-2024 | Streptococci | Original Article

Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique

A randomized split-mouth study

Authors: Raquel Fernanda Bachiega Morelli, Paulo Nelson-Filho, Karla Carpio Horta, Magda Feres, José Tarcísio Lima Ferreira, Fábio Lourenço Romano, Maria Bernadete Sasso Stuani, Maria Conceição Pereira Saraiva, Caio Luiz Bitencourt Reis, Mirian Aiko Nakane Matsumoto

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

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Abstract

Objective

The aim of this study is to assess the microbial contamination of three different brands of esthetic elastomeric ligatures.

Materials and methods

Different brands of esthetic ligatures (Unistick Pearl [American Orthodontics, Sheboygan, WI, USA], Power Sticks Pearl [Ortho Technology, Tampa, FL, USA], and Ease [Obscure, 3M Unitek, Monrovia, CA, USA]) were randomly assigned to permanent canines of 25 patients (aged 11–18 years) undergoing corrective orthodontic treatment. After 30 days, the ligatures were removed, processed, and the biofilm composition was analyzed by checkerboard DNA–DNA hybridization for 40 bacterial species. The microbiological data were analyzed using a nonparametric mixed model.

Results

The ligatures presented intense microbial contamination after 30 days, but no statistically significant differences were observed among the three groups (p > 0.05). The levels of the evaluated individual species and proportions of the microbial complexes showed no statistically significant differences among the ligature groups (p > 0.05).

Conclusions

Esthetic elastomeric ligatures became multicolonized by several bacterial species after 30 days of exposure to the oral cavity. However, no relevant differences were observed among the biofilm composition formed on the different ligature brands.
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Metadata
Title
Microbial contamination profile on esthetic elastomeric ligatures through the checkerboard DNA–DNA hybridization technique
A randomized split-mouth study
Authors
Raquel Fernanda Bachiega Morelli
Paulo Nelson-Filho
Karla Carpio Horta
Magda Feres
José Tarcísio Lima Ferreira
Fábio Lourenço Romano
Maria Bernadete Sasso Stuani
Maria Conceição Pereira Saraiva
Caio Luiz Bitencourt Reis
Mirian Aiko Nakane Matsumoto
Publication date
05-01-2024
Publisher
Springer Medizin
Keyword
Streptococci
Published in
Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI
https://doi.org/10.1007/s00056-023-00507-w