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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

Published in:

01-03-2017 | Original Article

Histologic and histomorphometric evaluation of bone regeneration using nanocrystalline hydroxyapatite and human freeze-dried bone graft

An experimental study in rabbit

Authors: Rokhsareh Sadeghi, Mohammad Najafi, Hassan Semyari, Fatemeh Mashhadiabbas

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Issue 2/2017

Abstract

Purpose

Bone regeneration is an important concern in periodontal treatment and implant dentistry. Different biomaterials and surgical techniques have been used for this purpose. The aim of the present study was to compare the effect of nanocrystalline hydroxyapatite and human freeze-dried bone graft (FDBG) in regeneration of rabbit calvarium bony defects by histologic and histomorphometric evaluation.

Methods

In this experimental study, three similar defects, measuring 8 mm in diameter, were created in the calvaria of 16 white New Zealand rabbits. Two defects were filled with FDBG and nanocrystalline hydroxyapatite silica gel, while the other one remained unfilled to be considered as control. All the defects were covered with collagen membranes. During the healing period, two animals perished; so 14 rabbits were divided into two groups: half of them were euthanized after 6 weeks of healing and the other half after 12 weeks. The specimens were subjected to histologic and histomorphometric examinations for assessment of the following variables: percentage of bone formation and residual graft material, inflammation scores, patterns of bone formation and type of newly formed bone.

Results

The percentages of new bone formation after 6 weeks were 14.22 ± 7.85, 21.57 ± 6.91, and 20.54 ± 10.07% in FDBG, NanoBone, and control defects. These values were 27.54 ± 20.19, 23.86 ± 6.27, and 26.48 ± 14.18% in 12-week specimens, respectively. No significant differences were found in the amount of bone formation between the groups. With regard to inflammation, the control and NanoBone groups showed significantly less inflammation compared to FDBG at the 6-week healing phase (P = 0.04); this difference was not significant in the 12-week specimens.

Conclusions

Based on the results of this experimental study, both NanoBone and FDBG exhibited a similar effect on bone formation.

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Title: Histologic and histomorphometric evaluation of bone regeneration using nanocrystalline hydroxyapatite and human freeze-dried bone graft
An experimental study in rabbit
Authors: Rokhsareh Sadeghi
Mohammad Najafi
Hassan Semyari
Fatemeh Mashhadiabbas
Publication date: 01-03-2017
Publisher: Springer Medizin
Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Issue 2/2017
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-016-0067-8

At a glance: The STEP trials

Springer Medicine

Histologic and histomorphometric evaluation of bone regeneration using nanocrystalline hydroxyapatite and human freeze-dried bone graft

An experimental study in rabbit

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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