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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

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01-11-2013 | Original article

Three-dimensional analysis of initial biofilm formation on polytetrafluoroethylene in the oral cavity

Authors: C. Fuchslocher Hellemann, S. Grade, W. Heuer, M.P. Dittmer, M. Stiesch, R. Schwestka-Polly, PD Dr. A.P. Demling

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Issue 6/2013

Abstract

Aim

There is published evidence that polytetrafluoroethylene (PTFE) exhibits beneficial surface characteristics by means of long-term biofilm accumulation. The purpose of this study was to investigate and compare early biofilm formation on polytetrafluoroethylene, ceramic-reinforced polytetrafluoroethylene and as the control group, stainless steel.

Materials and methods

This study comprised 10 healthy volunteers (5 females and 5 males) with a mean age of 27.3±3.7 years. Three different slabs (two PTFE coatings: one pure and one ceramic-reinforced polytetrafluoroethylene, and stainless steel) were placed in random order on a splint in the mandibular molar region. Intraoral splints were inserted for 48 h. After 48 h, we removed the slabs from the splints and stained the biofilm with a two-color fluorescence assay for bacterial viability (LIVE/DEAD BacLight—Bacterial Viability Kit 7012, Invitrogen, Mount Waverley, Australia). The amount of biofilm accumulation was assessed using confocal laser scanning microscopy (CLSM).

Results

The biofilm surface coverage was 55.8±39.8% on pure PTFE-coated probes, 55.9±35.0% on ceramic-reinforced PTFE-coated probes, and 33.3±37.8% on stainless steel. The differences among the three groups were not significant (p=0.301). Biofilm depth was 5.6±5.4 μm on pure PTFE-coated probes, 5.2±3.8 μm on ceramic-reinforced PTFE-coated probes, and 2.4±2.9 μm on stainless steel. The Friedman test revealed a significant difference in biofilm depth (p=0.002). Pairwise comparison of biofilm accumulation yielded a significant difference between pure PTFE and ceramic-reinforced PTFE compared to stainless steel (p=0.017; p=0.005).

Conclusion

Our results indicate that the beneficial surface characteristics of PTFE coatings by reducing long-term biofilm are not a result of inhibiting initial bacterial adhesion.

Ahn SJ, Lim BS, Lee SJ (2007) Prevalence of cariogenic streptococci on incisor brackets detected by polymerase chain reaction. Am J Orthod Dentofacial Orthop 131:736–741PubMedCrossRef

Al Shamsi AH, Cunningham JL, Lamey PJ et al (2007) Three-dimensional measurement of residual adhesive and enamel loss on teeth after debonding of orthodontic brackets: an in-vitro study. Am J Orthod Dentofacial Orthop 131:301.e9–e15

Arweiler NB, Hellwig E, Sculean A et al (2004) Individual vitality pattern of in situ dental biofilms at different locations in the oral cavity. Caries Res 38:442–447PubMedCrossRef

Auschill TM, Arweiler NB, Brecx M et al (2002) The effect of dental restorative materials on dental biofilm. Eur J Oral Sci 110:48–53PubMedCrossRef

Auschill TM, Hellwig E, Sculean A et al (2004) Impact of the intraoral location on the rate of biofilm growth. Clin Oral Invest 8:97–101CrossRef

Berezow AB, Darveau RP (2000) Microbial shift and periodontitis. Periodontol 55:36–47CrossRef

Berry JA, Biedlingmaier JF, Whelan PJ (2000) In vitro resistance to bacterial biofilm formation on coated fluoroplastic tympanostomy tubes. Otolaryngol Head Neck Surg 123:246–251PubMedCrossRef

Bollen CM, Papaioannou W, Van Eldere J et al (1996) The influence of abutment surface roughness on plaque accumulation and peri-implant mucositis. Clin Oral Implants Res 7:201–211PubMedCrossRef

Darveau RP (2009) The oral microbial consortium’s interaction with the periodontal innate defense system. DNA Cell Biol 28:389–395PubMedCentralPubMedCrossRef

10.

Demling A, Elter C, Heidenblut T et al (2010) Reduction of biofilm on orthodontic brackets with the use of polytetrafluoroethylene coating. Eur J Orthod 32:414–418PubMedCrossRef

11.

Elter C, Heuer W, Demling A et al (2011) Comparative analysis of biofilm formation on dental implant abutments with respect to supra- and subgingival areas: polytetrafluoroethylene versus titanium. Int J Prosthodont 24:373–375PubMed

12.

Hannig M (1999) Transmission electron microscopy of early plaque formation on dental materials in vivo. Eur J Oral Sci 107:55–64PubMedCrossRef

13.

Lang NP, Adler R, Joss A et al (1990) Absence of bleeding on probing. An indicator of periodontal stability. J Clin Periodontol 17:714–721PubMedCrossRef

14.

Lee SM, Yoo SY, Kim HS et al (2005) Prevalence of putative periodontopathogens in subgingival dental plaque from gingivitis lesions in Korean orthodontic patients. J Microbiol 43:260–265PubMed

15.

Lo Bue AM, Di Marco R, Milazzo I et al (2008) Microbiological and clinical periodontal effects of fixed orthodontic appliances in pediatric patients. New Microbiol 3:299–302

16.

Müller C, Lüders A, Hoth-Hannig W et al (2010) Initial bioadhesion on dental materials as a function of contact time, pH, surface wettability, and isoelectric point. Langmuir 26:4136–4141PubMedCrossRef

17.

Naranjo AA, Triviño ML, Jaramillo A et al (2006) Changes in the subgingival microbiota and periodontal parameters before and 3 months after bracket placement. Am J Orthod Dentofacial Orthop 130:275.e17–e22PubMedCrossRef

18.

Netuschil L, Reich E, Unteregger G et al (1998) A pilot study of confocal laser scanning microscopy for the assessment of undisturbed dental plaque vitality and topography. Arch Oral Biol 43:277–285PubMedCrossRef

19.

Ogaard B (1989) Prevalence of white spot lesions in 19-year-olds: a study on untreated and orthodontically treated persons 5 years after treatment. Am J Orthod Dentofacial Orthop 96:423–427PubMedCrossRef

20.

Paolantonio M, di Girolamo G, Pedrazzoli V et al (1996) Occurrence of Actinobacillus actinomycetemcomitans in patients wearing orthodontic appliance. A cross-sectional study. J Clin Periodontol 23:112–118PubMedCrossRef

21.

Paolantonio M, Festa F, di Placido G et al (1999) Site-specific subgingival colonization by Actinobacillus actinomycetemcomitans in orthodontic patients. Am J Orthod Dentofacial Orthop 115:423–428PubMedCrossRef

22.

Quirynen M, Bollen CM, Papaioannou W et al (1996) The influence of titanium abutment surface roughness on plaque accumulation and gingivitis: short-term observations. Int J Oral Maxillofac Implants 11:169–178PubMed

23.

Ristic M, Vlahovic Svabic M, Sasic M et al (2007) Clinical and microbiological effects of fixed orthodontic appliances on periodontal tissues in adolescents. Orthod Craniofac Res 10:187–195PubMedCrossRef

24.

Rupp F, Axmann D, Ziegler C et al (2002) Adsorption/desorption phenomena on pure and Teflon AF-coated titania surfaces studied by dynamic contact angle analysis. J Biomed Mater Res 62:567–578PubMedCrossRef

25.

Sallum EJ, Nouer DF, Klein MI et al (2004) Clinical and microbiologic changes after removal of orthodontic appliances. Am J Orthod Dentofacial Orthop 126:363–366PubMedCrossRef

26.

Silness J, Löe H (1964) Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 22:121–135PubMedCrossRef

27.

Stratmann U, Schaarschmidt K, Wegener H et al (1996) The extent of enamel surface fractures. A quantitative comparison of thermally debonded ceramic and mechanically debonded metal brackets by energy dispersive micro- and image-analysis. Eur J Orthod 18:655–662PubMed

28.

Türkkahraman H, Sayin MO, Bozkurt FY et al (2005) Archwire ligation techniques, microbial colonization, and periodontal status in orthodontically treated patients. Angle Orthod 75:231–236PubMed

29.

van Gastel J, Quirynen M, Teughels W et al (2008) Longitudinal changes in microbiology and clinical periodontal variables after placement of fixed orthodontic appliances. J Periodontol 79:2078–2086CrossRef

30.

van Gastel J, Quirynen M, Teughels W et al (2011) Longitudinal changes in microbiology and clinical periodontal parameters after removal of fixed orthodontic appliances. Eur J Orthod 33:15–21CrossRef

31.

Wiechmann D (2002) A new bracket system for lingual orthodontic treatment. Part 1: theoretical background and development. J Orofac Orthop 63:234–245PubMedCrossRef

32.

Wiechmann D (2003) A new bracket system for lingual orthodontic treatment. Part 2: first clinical experiences and further development. J Orofac Orthop 64:372–388PubMedCrossRef

33.

Wiechmann D, Rummel V, Thalheim A et al (2003) Customized brackets and archwires for lingual orthodontic treatment. Am J Orthod Dentofacial Orthop 124:593–599PubMedCrossRef

34.

Wood SR, Kirkham J, Marsh PD et al (2000) Architecture of intact natural human plaque biofilms studied by confocal laser scanning microscopy. J Dent Res 79:21–27PubMedCrossRef

Title: Three-dimensional analysis of initial biofilm formation on polytetrafluoroethylene in the oral cavity
Authors: C. Fuchslocher Hellemann
S. Grade
W. Heuer
M.P. Dittmer
M. Stiesch
R. Schwestka-Polly
PD Dr. A.P. Demling
Publication date: 01-11-2013
Publisher: Springer Berlin Heidelberg
Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Issue 6/2013
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-013-0174-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Three-dimensional analysis of initial biofilm formation on polytetrafluoroethylene in the oral cavity

Abstract

Aim

Materials and methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Aim

Materials and methods

Results

Conclusion

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