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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

Published in:

01-07-2011 | Original article

Prevalence of Helicobacter pylori in the adolescent oral cavity

Dependence on orthodontic therapy, oral flora and hygiene

Authors: Prof. Dr. A. Wichelhaus, L. Brauchli, Q. Song, G. Adler, G. Bode

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Issue 3/2011

Abstract

Background

Helicobacter pylori (HP) infection usually occurs in childhood. While there are various studies on the prevalence of HP in dental plaque, ours is the first to analyze its prevalence during orthodontic therapy and its interaction with competitive bacteria in adolescents.

Subjects and methods

The prevalence of HP was examined before and during the first 12 weeks of orthodontic therapy with fixed appliances in 11 patients with a mean age of 12.7 years. A total of 93 plaque samples were analyzed using PCR. The data acquired at every consultation were the following: PCR analysis of dental plaque and ¹³C urea breath tests for HP, quantitative analyses of saliva for Lactobacilli and Streptococcus mutans, the interproximal plaque index (API), and sulcus bleeding index (SBI).

Results

The prevalence of HP in plaque was 82% before orthodontic therapy, dropping to 54% during therapy (t test, p≤0.05). In contrast to HP’s prevalence, the Lactobacilli count rose (p≤0.05). The number of Streptococcus mutans bacteria in saliva decreased during orthodontic therapy (p≤0.05).

Conclusion

The prevalence of HP in dental plaque amounted to 82%. Orthodontic treatment did not reduce its prevalence. The prevalence of Lactobacilli was inversely proportional to that of HP.

Al-Hawajri AA, Keret D, Simhon A et al (2004) Helicobacter pylori DNA in dental plaques, gastroscopy, and dental devices. Dig Dis Sci 49:1091–1094PubMedCrossRef

Allaker RP, Young KA, Hardie JM et al (2002) Prevalence of Helicobacter pylori at oral and gastrointestinal sties in children: evidence for possible oral-to-oral transmission. J Med Microbiol 51:312–317PubMed

Ashorn M (1995) What are the specific features of Helicobacter pylori gastritis in children? Ann Med 27:617–620PubMedCrossRef

Attin R, Thon C, Schlagenhauf U et al (2005) Recolonization of mutans steptococci on teeth with orthodontic appliances after antimicrobial therapy. Eur J Orthod 27:489–493PubMedCrossRef

Banatvala N, Romero Lopez C, Owen RI et al (1994) Use of the polymerase chain reaction to detect Helicobacter pylori in the dental plaque of healthy and symptomatic individuals. Mircob Ecol Health Dis 7:1–8CrossRef

Braden B, Schäfer F, Caspary WF et al (1996) Nondispersive isotope-selective infrared spectroscopy: a new analytical method for ¹³C-urea breath tests. Scand J Gastroenterol 31:442–445PubMedCrossRef

Brenner H, Rothenbacher D, Bode G et al (1998) Parental history of gastric or duodenal ulcer and prevalence of Helicobacter pylori infection in preschool children: population based study. BMJ 316:665PubMed

Brown LM (2000) Helicobacter pylori: Epidemiology and routes of transmission. Epidemiol Rev 22:283–297PubMed

Cellini L, Allocati N, Piattelli A et al (1995) Microbiological evidence of Helicobacter pylori from dental plaque in dyspeptic patients. New Microbiol 18:187–192PubMed

10.

Chang HS, Walsh LJ, Freer TJ (1999) The effect of orthodontic treatment on salivary flow, pH, buffer capacity, and levels of mutans streptococci and lactobacilli. Aust Orthod J 15:229–234PubMed

11.

Chaudhry S, Iqbal HA, Khan AA et al (2008) Helicobacter pylori in dental plaque and gastric mucosa: correlation revisited. J Pak Med Assoc 58:331–334PubMed

12.

Cheng LH, Webberley M, Evans M et al (1996) Helicobacter pylori in dental plaque and gastric mucosa. Oral Surg Oral Med Oral Pathol Oral Radio Endod 81:421–423CrossRef

13.

Chumpitaz Conde J, Guitiérrez Manay, Córdova Acosta R et al (2006) Isolation of helicobacter pylori in dental plaque in patients with gastritis and “Angamos” clinic. Rev Gastroenterol Peru 26:373–376

14.

Ellenrieder V, Glasbrenner B, Stoffels C et al (1997) Qualitative and semi-quantitative value of a modified ¹³C-urea breath test for identification of Helicobacter pylori infection. Eur J Gastroenterol Hepatol 9:1085–1089PubMed

15.

Ferguson DA Jr, Li C, Patel NR et al (1993) Isolation of Helicobacter pylori from saliva. J Clin Microbiol 31:2802–2804PubMed

16.

Grimm W, Fischbach W (2003) Helicobacter pylori infection in children and juveniles: an epidemiological study on prevalence, socio-economic factors and symptoms. (Abstract) Dtsch Med Wochenschr 128:1878–1883

17.

Hardo PG, Tugnait A, Hassan F et al (1995) Helicobacter pylori infection and dental care. Gut 37:44–46PubMedCrossRef

18.

Ishihara K, Miura T, Kimizuka R et al (1997) Oral bacteria inhibit Helicobacter pylori growth. FEMS Microbiol Lett 152:355–361PubMedCrossRef

19.

Krajden S, Fuksa M, Anderson J et al (1989) Examination of human stomach biopsies, saliva and dental plaque for Campylobacter pylori. J Clin Microbial 31:1397–1398

20.

Lange DE, Plagman HC, Eenboom A et al (1997) Klinische Bewertungsverfahren zur Objektivierung der Mundhygiene. Dtsch Zahnarztl Z 32:44–47

21.

Leung WK, Sung JJ, Ling TK et al (1999) Use of chopsticks for eating and Helicobacter pylori infection. Dig Dis Sci 44:1173–1176PubMedCrossRef

22.

Li C, Ha T, Ferguson DAJ et al (1996) A newly developed PCR assay of H. pylori in gastric biopsy, saliva, and feces. Evidence of high prevalence of H. pylori in saliva supports oral transmission. Dig Dis Sci 41:2142–2149PubMedCrossRef

23.

Li C, Musich PR, Ha T et al (1995) High prevalence of Helicobacter pylori in saliva demonstrated by a novel PCR assay. J Clin Pathol 48:662–666PubMedCrossRef

24.

Liu Y, Yue H, Li A et al (2009) An epidemiologic study on the correlation between oral Helicobacter pylori and gastric H. pylori. Curr Microbiol 58:449–453PubMedCrossRef

25.

Ma JL, You WC, Gail MH et al (1998) Helicobacter pylori infection and mode of transmission in a population at high risk of stomach cancer. Int J Epidemiol 27:570–573PubMedCrossRef

26.

Madinier IM, Fosse TM, Monteil RA (1997) Oral carriage of Helicobacter pylori: a review. J Periodontol 68:2–6PubMed

27.

Mapstone NP, Lynch DA, Lewis FA et al (1993) Identification of Helicobacter pylori DANN in the mouths and stomachs of patients with gastritis usign PCR. J Clin Pathol 46:540–543PubMedCrossRef

28.

Marshall BJ, Warren JR (1984) Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 323:1311–1315CrossRef

29.

Mühlemann HR, Son S (1971) Gingival sulcus bleeding-a leading symptom in initial gingivitis. Helv Odontol Acta 15:107–113PubMed

30.

Nabwera HM, Logan RP (1999) Epidemiology of Helicobacter pylori: transmission, translocation and extragastric reservoirs. J Physiol Pharmacol 50:711–722PubMed

31.

Namavar F, Roosendaal R, Kuipers EJ et al (1995) Presence of Helicobacter pylori in the oral cavity, oesophagus, stomach and faeces of patients with gastritis. Eur J Clin Microbiol Infect Dis 14:234–237PubMedCrossRef

32.

Oshowo A, Gillam D, Botha A et al (1998) Helicobacter pylori: the mouth, stomach, and gut axis. Ann Periodontol 3:276–280PubMedCrossRef

33.

Peach HG, Pearce DC, Farish SJ (1997) Helicobacter pylori infection in an Australian regional city: prevalence and risk factors. Med J Aust 167:310–313PubMed

34.

Pustorino R, Nicosia R, D’Ambra G et al (1996) The mouth-stomach crossing of Helicobacter pylori. Riv Eur Sci Med Farmacol 18:183–186PubMed

35.

Shimada T, Ogura K, Ota S et al (1994) Identification of Helicobacter pylori in gastric specimens, gastric juice, saliva, and faeces of Japanese patients. (Letter). Lancet 343:1636–1637PubMedCrossRef

36.

Warren JR, Marshall B (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis. (Letter). Lancet 321:1273–1375CrossRef

37.

Ying L, Huanjian L, Yang B et al (2008) Study on the relationship between Helicobacter pylori in the dental plaque and the occurrence of dental caries or oral hygiene index. Helicobacter 13:256–260CrossRef

38.

Young KA, Allaker RP, Hardie JM (2001) Morphological analysis of Helicobacter pylori from gastric biopsies and dental plaque by scanning electron microscopy. Oral Microbiol Immunol 16:178–181PubMedCrossRef

Title: Prevalence of Helicobacter pylori in the adolescent oral cavity
Dependence on orthodontic therapy, oral flora and hygiene
Authors: Prof. Dr. A. Wichelhaus
L. Brauchli
Q. Song
G. Adler
G. Bode
Publication date: 01-07-2011
Publisher: Springer-Verlag
Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Issue 3/2011
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-011-0024-5

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Prevalence of Helicobacter pylori in the adolescent oral cavity

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Background

Subjects and methods

Results

Conclusion

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