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Lasers in Dental Science

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01-03-2018 | Original Article

The antibacterial effect of the combined Er,Cr:YSGG and 940 nm diode laser therapy in treatment of periodontitis: a pilot study

Authors: C. Ciurescu, L. Vanweersch, R. Franzen, N. Gutknecht

Published in: Lasers in Dental Science | Issue 1/2018

Abstract

Aim

The aim of the study was to accurately assess the antibacterial effect of the combined Er,Cr:YSGG and InGaAsP 940 nm laser therapy on nine pathogenic bacteria in the treatment of periodontitis.

Materials and method

Fifty-six patients were selected for this pilot study. Five patients were excluded, whereas 51 of them completed the study. The patients were randomly allocated to either the combined 2780 nm Er,Cr:YSGG (Waterlase, Biolase) and 940 nm InGaAsP diode laser (EPIC, Biolase) therapy, adjunct to scaling and root planning (SRP) (experimental group), or to scaling and root planning alone (control group). The quantitative and qualitative analysis of the total number of bacteria and nine specific germs was performed using quantitative real-time polymerase chain reaction.

Results

The total bacterial load inside the periodontal pockets was reduced both for the laser plus SRP and for the SRP alone group at the 1-month and 6-month follow-ups (p < 0.05). The laser therapy group showed a more significant bacterial reduction than the control group at the 1-month and 6-month follow-ups. The germ number reduction was statistically strongly significant for the total number of germs and for eight out of nine analyzed bacteria.

Conclusions

The present study suggests that a combined Er,Cr:YSGG 2780 nm and diode InGaAsP 940 nm laser therapy added to the nonsurgical periodontal treatment brings an important benefit in bacterial reduction and stands as a reliable alternative to antibiotic prescriptions in periodontal treatment. The positive changes are also reflected in significant improvement of clinical periodontal parameters. The results suggest that newly formed bacterial microbiome inside the sulcus appears to be more beneficial, durable, and stable in the lased group.

Demmer R, Papapanou P (2010) Epidemiologic patterns of chronic and aggressive periodontitis. Periodontol 2000 53(1):28–44. https://doi.org/10.1111/j.1600-0757.2009.00326.x CrossRefPubMedPubMedCentral

Teles R, Teles F, Frias-Lopez J, Paster B, Haffajee A (2013) Lessons learned und unlearned in periodontal microbiology. Periodontol 62(1):95–162. https://doi.org/10.1111/prd.12010 CrossRef

Lappin-Scott HM, Bass C (2001) Biofilm formation: attachment, growth, and detachment of microbes from surface. Am J Infect Control 29(4):250–251. https://doi.org/10.1067/mic.2001.115674 CrossRefPubMed

Haffajee AD, Patel M, Socransky SS (2008) Microbiological changes associated with four different periodontal therapies for the treatment of chronic periodontitis. Oral Microbiol Immunol 23(2):148–157. https://doi.org/10.1111/j.1399-302X.2007.00403.x CrossRefPubMed

Heitz-Mayfield LJ, Trombelli L, Heitz F, Needleman I, Moles D (2002) A systematic review of the effect of surgical debridement vs non-surgical debridement for the treatment of chronic periodontitis. J Clin Periodontol 29(Suppl 3):92–102. https://doi.org/10.1034/j.1600-051X.29.s3.5.x CrossRefPubMed

Darveau RP (2010) Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microb 8(7):481–490. https://doi.org/10.1038/nrmicro2337 CrossRef

Suzuki N, Yoneda M, Hirofuji T (2013) Mixed red-complex bacterial infection in periodontitis. Int J Dent 587279. Published online 2013 Mar 6. https://doi.org/10.1155/2013/587279

Bodet C, Chandad F, Grenier D (2007) Pathogenic potential of Porphyromonas gingivalis, Treponema denticola and Tannarella forsythia, the red bacterial complex associated with periodontitis. Pathol Biol (Paris) 55(3–4):154–162. https://doi.org/10.1016/j.patbio.2006.07.045 CrossRef

Johnson JD, Chen R, Lenton PA, Zhang G, Hinrichs JE, Rudney JD (2008) Persistence of extracrevicular bacterial reservoirs after treatment of aggressive periodontitis. J Clin Periodontol 79(12):2305–2312. https://doi.org/10.1902/jop.2008.080254 CrossRef

10.

Martelli FS, Fanti E, Rosati C, Martelli M, Bacci G, Martelli ML, Medico E (2016) Long-term efficacy of microbiology driven periodontal laser assisted therapy. Eur J Clin Microbiol Dis 35(3):423–431. https://doi.org/10.1007/s10096-015-2555-y CrossRef

11.

Al-Falaki R, Hughes FJ, Wadia R (2016) Minimally invasive treatment of infrabony periodontal defects using dual-wavelength laser therapy. Int Sch Res Notices 25(2):131–139

12.

Gojkov-Vukelic M, Hadzic S, Dedic A, Konjhodzic R, Beslagic E (2013) Application of a diode laser in the reduction of targeted periodontal pathogens. Acta Inform Med 21(4):237–240. https://doi.org/10.5455/aim.2013.21.237-240 CrossRefPubMedPubMedCentral

13.

Gutknecht N, Van Betteray C, Ozturan S, Vanweersch L, Franzen R (2015) Laser supported reduction of specific microorganisms in the periodontal pocket with the aid of an Er,Cr:YSGG laser: a pilot study. Sci World J 450258. https://doi.org/10.1155/2015/450258

14.

Crispino A, Figliuzzi MM, Iovane C, DelGiudice T, Lomnano S, Pacifico D, Fortunato L, Del Giudice R (2015) Effectiveness of a diode laser in addition to non-surgical periodontal therapy: study of intervention. Anm Sromatologica (Roma) 6(1):15–20 Published online May 2015

15.

Cobb CM (2006) Lasers in periodontics:a review of the literature. J Periodontol 77(4):545–564. https://doi.org/10.1902/jop.2006.050417 CrossRefPubMed

16.

Euzebio Alves VT, de Andrade AK, Toaliar JM, Conde MC, Zezell DM, Cai S, Pannuti CM, De Micheli G (2013) Clinical and microbiological evaluation of high intensity diode laser adjutant to non-surgical periodontal treatment: a 6-month clinical trial. Clin Oral Investig 17(1):87–95. https://doi.org/10.1007/s00784-012-0703-7 CrossRefPubMed

17.

Caruso U, Nastri L, Piccolomini R, d’Ercole S, Mazza C, Guida L (2008) Use of diode laser 980 nm as an adjunctive therapy in the treatment of chronic periodontitis. A randomized controlled clinical trial. New Microbiol 31(4):513–518PubMed

18.

Cugini MA, Haffajee AD, Smith C, Kent RL Jr, Socransky SS (2000) The effect of scaling and root planing on the clinical and microbiological parameters of periodontal diseases: 12-month results. J Clin Periodontol 27(1):30–36. https://doi.org/10.1034/j.1600-051x.2000.027001030.x CrossRefPubMed

19.

Van Steenbergen TJ, Timmerman MF, Mikx FH, de Quincey G, van der Weijden GA, van der Velden U, de Graaff J (1996) Discrepancy between culture and DNA probe analysis for the detection of periodontal bacteria. J Clin Periodontol 23(10):955–959. https://doi.org/10.1111/j.1600-051X.1996.tb00518.x CrossRefPubMed

20.

Tomasi C, Leyland AH, Wennstrom JL (2007) Factors influencing the outcome of non-surgical periodontal treatment: a multilevel approach. J Clin Periodontol 34(8):682–690. https://doi.org/10.1111/j.1600-051X.2007.01111.x CrossRefPubMed

21.

Ximenez-Fyvie LA, Haffajee AD, Som S, Thompson M, Torresyap G, Socransky SS (2000) The effect of repeated professional supragingival plaque removal on the composition of the supra- and subgingival microbiota. J Clin Periodontol 27(9):637–647. https://doi.org/10.1034/j.1600-051x.2000.027009637.x CrossRefPubMed

22.

Paolantonio M, D’Angelo M, Grassi RF, Perinetti G, Piccolomini R, Pizzo G, Annunziata M, D’Archivio D, D’Ercole S, Nardi G, Guida L (2008) Clinical and microbiologic effects of subgingival controlled-release delivery of chlorhexidine chip in the treatment of periodontitis: a multicenter study. J Periodontol 79(2):271–282. https://doi.org/10.1902/jop.2008.070308 CrossRefPubMed

23.

Checchi L, Montevecchi M, Gatto MR, Trombelli L (2002) Retrospective study of tooth loss in 92 treated periodontal patients. J Clin Periodontol 29(7):651–656. https://doi.org/10.1034/j.1600-051X.2002.290710.x CrossRefPubMed

24.

Hellstrom MK, Ramberg P, Krok L, Lindhe J (1996) The effect of supragingival plaque control on the subgingival microflora in human periodontitis. J Clin Periodontol 23(10):934–940. https://doi.org/10.1111/j.1600-051X.1996.tb00514.x CrossRefPubMed

25.

Feres M, Haffajee AD, Allard K, Som S, Socransky SS (2001) Change in subgingival microbial profiles in adult periodontitis subjects receiving either systemically-administered amoxicillin or metronidazole. J Clin Periodontol 28(7):597–609. https://doi.org/10.1034/j.1600-051x.2001.028007597.x CrossRefPubMed

Title: The antibacterial effect of the combined Er,Cr:YSGG and 940 nm diode laser therapy in treatment of periodontitis: a pilot study
Authors: C. Ciurescu
L. Vanweersch
R. Franzen
N. Gutknecht
Publication date: 01-03-2018
Publisher: Springer International Publishing
Published in: Lasers in Dental Science / Issue 1/2018
Electronic ISSN: 2367-2587
DOI: https://doi.org/10.1007/s41547-017-0018-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The antibacterial effect of the combined Er,Cr:YSGG and 940 nm diode laser therapy in treatment of periodontitis: a pilot study

Abstract

Aim

Materials and method

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Aim

Materials and method

Results

Conclusions

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