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01-12-2015

JC virus urinary excretion and seroprevalence in natalizumab-treated multiple sclerosis patients

Authors: Serena Delbue, Francesca Elia, Camilla Carloni, Valentina Pecchenini, Diego Franciotta, Matteo Gastaldi, Elena Colombo, Lucia Signorini, Silvia Carluccio, Anna Bellizzi, Roberto Bergamaschi, Pasquale Ferrante

Published in: Journal of NeuroVirology | Issue 6/2015

Abstract

The risk of developing progressive multifocal leukoencephalopathy (PML), as a consequence of infection/reactivation with JC virus (JCV), is consistent in natalizumab-treated multiple sclerosis (MS) patients, with 430 cases of PML reported so far. The risk of PML is higher in JCV seropositive patients, and it is recommended that only MS patients without JCV antibodies should be enrolled in the treatment postulating that they do not have JCV infection.

We have studied forty-two natalizumab-treated MS patients, and urine and blood were collected monthly for up to 60 months. JCV and BK virus (BKV) DNA presence was verified using quantitative real-time PCR assays, and serum anti-JCV antibodies were measured with the Stratify and/or Stratify DxSelect tests.

JCV and BKV DNA were not found in the blood samples, whereas they were found at least once in the urine of 21 of 42 (50 %) and of 25/42 (59.5 %) patients, respectively. JCV DNA urinary shedding increased up to month 24 of natalizumab treatment (45.2 %), and the effect of time was significant for JCV (p = 0.04), but not for BKV (p = 0.39). JCV viruria and seropositivity did not completely correlate, since three patients shedding JCV DNA in the urine were seronegative according to the serological tests.

The results indicated that natalizumab therapy may increase the rate of JCV urinary shedding. Additionally, we confirmed that the identification of JCV carriers cannot solely rely on serological tests, but sensitive methods for viral DNA detection should be adopted to more precisely identify the truly JCV uninfected cases.

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Title: JC virus urinary excretion and seroprevalence in natalizumab-treated multiple sclerosis patients
Authors: Serena Delbue
Francesca Elia
Camilla Carloni
Valentina Pecchenini
Diego Franciotta
Matteo Gastaldi
Elena Colombo
Lucia Signorini
Silvia Carluccio
Anna Bellizzi
Roberto Bergamaschi
Pasquale Ferrante
Publication date: 01-12-2015
Publisher: Springer US
Published in: Journal of NeuroVirology / Issue 6/2015
Print ISSN: 1355-0284
Electronic ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-014-0268-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

JC virus urinary excretion and seroprevalence in natalizumab-treated multiple sclerosis patients

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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