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01-12-2012

Human trigeminal ganglionic explants as a model to study alphaherpesvirus reactivation

Authors: Yevgeniy Azarkh, Nathan Bos, Don Gilden, Randall J. Cohrs

Published in: Journal of NeuroVirology | Issue 6/2012

Abstract

Varicella zoster virus (VZV) latency is characterized by limited virus gene expression and the absence of virus DNA replication. Investigations of VZV latency and reactivation have been hindered by the lack of an in vitro model of virus latency. Since VZV is an exclusively human pathogen, we used naturally infected human trigeminal ganglia (TG) obtained at autopsy to study virus latency. Herein, we report optimization of medium to maintain TG integrity as determined by histology and immunohistochemistry. Using the optimized culture medium, we also found that both herpes simplex virus-1 (HSV-1) and VZV DNA replicated in TG explants after 5 days in culture. The increase in HSV-1 DNA was fourfold greater than the increase in VZV DNA. Overall, we present a model for alphaherpesvirus latency in human neurons in which the key molecular events leading to virus reactivation can be studied.

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Title: Human trigeminal ganglionic explants as a model to study alphaherpesvirus reactivation
Authors: Yevgeniy Azarkh
Nathan Bos
Don Gilden
Randall J. Cohrs
Publication date: 01-12-2012
Publisher: Springer US
Published in: Journal of NeuroVirology / Issue 6/2012
Print ISSN: 1355-0284
Electronic ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-012-0123-0

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Human trigeminal ganglionic explants as a model to study alphaherpesvirus reactivation

Abstract

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