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Tumor Biology
Published in: Tumor Biology 9/2016

01-09-2016 | Original Article

Yeast two-hybrid screening identified WDR77 as a novel interacting partner of TSC22D2

Authors: Qiao Li, Pan Chen, Zhaoyang Zeng, Fang Liang, Yali Song, Fang Xiong, Xiayu Li, Zhaojian Gong, Ming Zhou, Bo Xiang, Cong Peng, Xiaoling Li, Xiang Chen, Guiyuan Li, Wei Xiong

Published in: Tumor Biology | Issue 9/2016

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Abstract

Transforming growth factor β-stimulated clone 22 domain family, member 2 (TSC22D2), a member of the TSC22D family, has been implicated as a tumor-associated gene, but its function remains unknown. To further explore its biological role, yeast two-hybrid screening combined with multiple bioinformatics tools was used to identify 44 potential interacting partners of the TSC22D2 protein that were mainly involved in gene transcription, cellular metabolism, and cell cycle regulation. The protein WD repeat domain 77 (WDR77) was selected for further validation due to its function in the cell cycle and tumor development, as well as its high detection frequency in the yeast two-hybrid assay. Immunoprecipitation and immunofluorescence experiments confirmed an interaction between the TSC22D2 and WDR77 proteins. Our work greatly expands the putative protein interaction network of TSC22D2 and provides deeper insight into the biological functions of the TSC22D2 and WDR77 proteins.
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Metadata
Title
Yeast two-hybrid screening identified WDR77 as a novel interacting partner of TSC22D2
Authors
Qiao Li
Pan Chen
Zhaoyang Zeng
Fang Liang
Yali Song
Fang Xiong
Xiayu Li
Zhaojian Gong
Ming Zhou
Bo Xiang
Cong Peng
Xiaoling Li
Xiang Chen
Guiyuan Li
Wei Xiong
Publication date
01-09-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 9/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-016-5113-z

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