Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Tumor Biology
Published in: Tumor Biology 5/2013

01-10-2013 | Research Article

Upregulated hPuf-A promotes breast cancer tumorigenesis

Authors: Chi-Chen Fan, Liek-Yeow Lee, Ming-Ying Yu, Chi-Yuan Tzen, Chi Chou, Mau-Sun Chang

Published in: Tumor Biology | Issue 5/2013

Login to get access

Abstract

hPuf-A is a member of RNA-binding PUF family that regulates mRNA translation. Redistribution of hPuf-A from the nucleolus to the nucleoplasm upon genotoxic stress modulates the poly(ADP-ribosyl)ation activity of PARP-1. Here, we report a novel function of hPuf-A involved in promoting breast cancer progression. Immunohistochemical studies showed higher expression levels of hPuf-A in stage I, II, III, and IV breast cancer specimens in contrast with those of hPuf-A in ductal carcinoma in situ. The presence of hPuf-A is highly associated with colony formation capacities in breast cancer T47D and MDA-MB-231 cells. Xenograft growth of hPuf-A-silenced and hPuf-A overexpressing MDA-MB-231 cells in nude mice was substantially in concert with colony formation capacities. This promoting effect of hPuf-A in tumorigenesis might be correlated with the regulation of its associated mRNAs, such as RbAp48 and DDX3. Collectively, hPuf-A may have diagnostic values in breast cancer progression.
Appendix
Available only for authorised users
Literature
1.
Wickens M, Bernstein DS, Kimble J, Parker R. A PUF family portrait: 3′UTR regulation as a way of life. Trends Genet. 2002;18:150–7.CrossRefPubMed
2.
Wang X, McLachlan J, Zamore PD, Hall TM. Modular recognition of RNA by a human Pumilio-homology domain. Cell. 2002;110:501–12.CrossRefPubMed
3.
4.
Zhang B, Gallegos M, Puoti A, Durkin E, Fields S, Kimble J, et al. A conserved RNA binding protein that regulates patterning of sexual fates in the C. elegans hermaphrodite germ line. Nature. 1997;390:477–84.CrossRefPubMed
5.
Zamore PD, Williamson JR, Lehmann R. The Pumilio protein binds RNA through a conserved domain that defines a new class of RNA-binding proteins. RNA. 1997;3:1421–33.PubMedCentralPubMed
6.
Miller MA, Olivas WM. Roles of Puf proteins in mRNA degradation and translation. Wiley Interdiscip Rev RNA. 2011;2:471–92.CrossRefPubMed
7.
Subramaniam K, Seydoux G. Dedifferentiation of primary spermatocytes into germ cell tumors in C. elegans lacking the Pumilio-like protein PUF-8. Curr Biol. 2003;13:134–9.CrossRefPubMed
8.
Kuo MW, Wang SH, Chang JC, Chang CH, Huang LJ, Lin HH, et al. A novel puf-A gene predicted from evolutionary analysis is involved in the development of eyes and primordial germ-cells. PLoS One. 2009;4:e4980.PubMedCentralCrossRefPubMed
9.
Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, Chang MS. hPuf-A/KIAA0020 modulates PARP-1 cleavage upon genotoxic stress. Cancer Res. 2011;71:1126–34.CrossRefPubMed
10.
Yoder BJ, Wilkinson EJ, Massoll NA. Molecular and morphologic distinctions between infiltrating ductal and lobular carcinoma of the breast. Breast J. 2007;13:172–9.CrossRefPubMed
11.
Carey LA, Perou CM, Livasy CA, Dressler LG, Cowan D, Conway K, et al. Race, breast cancer subtypes, and survival in the Carolina breast cancer study. Jama. 2006;295:2492–502.CrossRefPubMed
12.
Brickner AG, Warren EH, Caldwell JA, Akatsuka Y, Golovina TN, Zarling AL, et al. The immunogenicity of a new human minor histocompatibility antigen results from differential antigen processing. J Exp Med. 2001;193:195–206.PubMedCentralCrossRefPubMed
13.
Martello G, Rosato A, Ferrari F, Manfrin A, Cordenonsi M, Dupont S, et al. A microRNA targeting dicer for metastasis control. Cell. 2010;141:1195–207.CrossRefPubMed
14.
Lacroix M, Leclercq G. Relevance of breast cancer cell lines as models for breast tumours: an update. Breast Cancer Res Treat. 2004;83:249–89.CrossRefPubMed
15.
Harvey JM, Clark GM, Osborne CK, Allred DC. Estrogen receptor status by immunohistochemistry is superior to the ligand-binding assay for predicting response to adjuvant endocrine therapy in breast cancer. J Clin Oncol. 1999;17:1474–81.PubMed
16.
Rojo F, García-Parra J, Zazo S, Tusquets I, Ferrer-Lozano J, Menendez S, et al. Nuclear PARP-1 protein overexpression is associated with poor overall survival in early breast cancer. Ann Oncol. 2012;23:1156–64.CrossRefPubMed
17.
Botlagunta M, Vesuna F, Mironchik Y, Raman A, Lisok A, Winnard Jr P, et al. Oncogenic role of DDX3 in breast cancer biogenesis. Oncogene. 2008;27:3912–22.CrossRefPubMed
18.
Scuto A, Zhang H, Zhao H, Rivera M, Yeatman TJ, Jove R, et al. RbAp48 regulates cytoskeletal organization and morphology by increasing K-Ras activity and signaling through mitogen-activated protein kinase. Cancer Res. 2007;67:10317–24.CrossRefPubMed
Metadata
Title
Upregulated hPuf-A promotes breast cancer tumorigenesis
Authors
Chi-Chen Fan
Liek-Yeow Lee
Ming-Ying Yu
Chi-Yuan Tzen
Chi Chou
Mau-Sun Chang
Publication date
01-10-2013
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 5/2013
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-013-0801-4

Other articles of this Issue 5/2013

Tumor Biology 5/2013 Go to the issue

Research Article

Prognostic value of HMGB3 expression in patients with non-small cell lung cancer

Research Article

Overexpression of stathmin 1 is associated with poor prognosis of patients with gastric cancer

Research Article

Increased expression of FOXQ1 is a prognostic marker for patients with gastric cancer

Research Article

Prognostication of OCT4 isoform expression in prostate cancer

Research Article

TRAF6 promoted the tumorigenicity of esophageal squamous cell carcinoma

Research Article

A let-7 binding site polymorphism rs712 in the KRAS 3′ UTR is associated with an increased risk of gastric cancer
Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras
Prof. Fabrice Barlesi
Developed by: Springer Medicine
Image Credits