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Open Access 01-08-2012 | Research Article

Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines

Authors: Yuanyuan Ma, Dongming Liang, Jian Liu, Karol Axcrona, Gunnar Kvalheim, Karl-Erik Giercksky, Jahn M. Nesland, Zhenhe Suo

Published in: Tumor Biology | Issue 4/2012

Abstract

Bone marrow metastases are formed in the late phases of prostate cancer disease. Stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) are present in the microenvironment of the bone marrow and play a vital role in cell biology therein. The present study was to investigate the influence of SCF and G-CSF on stem-like properties in prostate cancer cell lines. Upon stimulation with SCF or G-CSF, higher levels of CD117, ABCG2, and CD44 were observed in PC-3 and DU145 cells examined by flow cytometry. Simultaneously, the expressions of Oct3/4 and Nanog were upregulated. Moreover, quantitative real-time PCR verified that the increased Nanog under the stimulations was mostly derived from NANOGP8. In parallel with the increasing expressions of these proteins, higher colony and sphere formation efficiencies were seen in these cells in response to the cytokine stimulations. Furthermore, a synergistic effect of SCF and G-CSF on colony and sphere formations and ABCG2 expression was disclosed. Our results indicate a favorable bone marrow niche for prostate cancer cells where higher levels of cell stemness are maintained at least partly by the cytokines SCF and G-CSF.

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Title: Synergistic effect of SCF and G-CSF on stem-like properties in prostate cancer cell lines
Authors: Yuanyuan Ma
Dongming Liang
Jian Liu
Karol Axcrona
Gunnar Kvalheim
Karl-Erik Giercksky
Jahn M. Nesland
Zhenhe Suo
Publication date: 01-08-2012
Publisher: Springer Netherlands
Published in: Tumor Biology / Issue 4/2012
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-012-0325-3

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