Top

Indian Journal of Hematology and Blood Transfusion

Published in:

13-01-2023 | ORIGINAL ARTICLE

Evaluation of a New Method of Leukocyte Extractions from the Leukoreduction Filter

Authors: Zahra Abbasi Malati, Ali Akbar Pourfathollah, Rasul Dabbaghi, Sahar Balagholi, Mohammad Reza Javan

Published in: Indian Journal of Hematology and Blood Transfusion | Issue 3/2023

Abstract

This study’s purpose was to optimize the leukocyte extraction protocol and evaluate the efficacy of this new protocol. 12BioR blood filters were collected from Tehran Blood Transfusion Center. A twosyringe system and Multi-step rinsing were designed for cell extraction. The final purpose of this optimization was: (1) removed the residual RBCs, (2) reversed the leukocyte trapping process, and (3) remove the microparticles to obtain the high yield of target cells. Finally, Extracted cells were evaluated by Automated Cell count; Samples smear differential cell count, Trypan blue, and Annexin-PI staining. The results showed that on average 11.88 × 10⁸ ± 3.32 leukocytes recovered after indirect washing and that the mean count of granulocytes, lymphocytes, and Monocyte in this sample was 5.24 ± 2.18 × 10⁸, 5.57 ± 1.74 × 10⁸, and 0.56 ± 0.38 × 10⁸ respectively. Also, the mean percent of manual differential cell count after concentration was 42.81%, 41.80%, and 15.82% for granulocytes, lymphocytes, and monocytes respectively. Moreover, viability and apoptosis assay showed > 95% viability in mononuclear cells recovered from LRFs. It is concluded that the use of a double-syringe system and RBC and microparticles removal from leukoreduction filters lead to acceptable viable leukocyte count that can be used in in vitro and in vivo studies.

Roback J, Combs M, Grossman B, Hillyer C (2008) American association of blood banks technical manual, vol 376. American Association of Blood Banks, Bethesda, MD, p 471

Sharma R, Marwaha N (2010) Leukoreduced blood components: advantages and strategies for its implementation in developing countries. Asian J Transfus Sci 4(1):3–8CrossRefPubMedPubMedCentral

Bianchi M, Vaglio S, Pupella S, Marano G, Facco G, Liumbruno GM, Grazzini G (2016) Leucoreduction of blood components: An effective way to increase blood safety? Blood Transfus 14(3):214PubMedPubMedCentral

Mönninghoff J, Moog R (2012) Investigation of a new in-lineleukocyte reduction filter for packed red blood cells. TransfusApher Sci 46(3):253–256

Singh S, Kumar A (2009) Leukocyte depletion for safe blood transfusion. Biotechnol J 4(8):1140–1151CrossRefPubMed

Singh S, Kumar A (2009) Leukocyte depletion for safe blood transfusion. Biotechnol J 4:1140–1151CrossRefPubMed

Fergusson D, Khanna MP, Tinmouth A, Hébert PC (2004) Transfusion of leukoreduced red blood cells may decrease postoperative infections: two meta-analyses of randomized controlled trials. Can J Anaesth 51(5):417–424CrossRefPubMed

Pennington J, Taylor GP, Sutherland J, Davis RE, Seghatchian J, Allain J-P et al (2002) Persistence of HTLV-I in blood components after leukocyte depletion. Blood 100(2):677–681CrossRefPubMed

Rapaille A, Moore G, Siquet J, Flament J, Sondag-Thull D (1997) Prestorage leukocyte reduction with in-line filtration of whole blood: evaluation of red cells and plasma storage. Vox Sang 73(1):28–35CrossRefPubMed

10.

Sen A, Khetarpal Sm A, Jetley S (2010) Comparative study of pre-deposit and bedside leucodepletion filters. Med J ArmedForces India 66(2):142–146

11.

Fujii Y (2016) The potential of the novel leukocyte removal filter in cardiopulmonary bypass. Expert Rev Med Devices 13(1):5–14CrossRefPubMed

12.

Bekeschus S, Kolata J, Winterbourn C, Kramer A, Turner R, Weltmann KD, Bröker B, Masur K (2014) Hydrogen peroxide: a central player in physical plasma-induced oxidative stress in human blood cells. Free Radic Res 48(5):542–549CrossRefPubMed

13.

Burton GW, Ingold KU, Thompson KE (1981) An improved procedure for the isolation of ghost membranes from human red blood cells. Lipids 16(12):946CrossRefPubMed

14.

Puren AJ, Fantuzzi G, Gu Y, Su MS, Dinarello CA (1998) Interleukin-18 (IFNgamma-inducing factor) induces IL-8 and IL-1beta via TNFalpha production from non-CD14+ human blood mononuclear cells. J Clin Investig 101(3):711–721CrossRefPubMedPubMedCentral

15.

Schindler R, Mancilla J, Endres S, Ghorbani R, Clark SC, Dinarello CA. Correlations and interactions in the production of interleukin-6 (IL-6), IL-1, and tumor necrosis factor (TNF) in human blood mononuclear cells: IL-6 suppresses IL-1 and TNF

16.

Meyer T, Zehnter I, Hofmann B, Zaisserer J, Burkhart J, Rapp S et al (2005) Filter buffy coats (FBC): a source of peripheral bloodleukocytes recovered from leukocyte depletion filters. J Immunol Methods 307(1–2):150–166CrossRefPubMed

17.

Sasani N, Roghanian R, Emtiazi G, Jalali SM, Zarif MN, Aghaie A (2020) Design and introduction of a rational mechanical eluting system for leukocyte recovery from leukoreduction filters: a cell differential approach. Transfus Clin Biol 27(3):172–178CrossRefPubMed

18.

Wegehaupt AK, Roufs EK, Hewitt CR, Killian ML, Gorbatenko O, Anderson CM, Killian MS (2017) Recovery and assessment of leukocytes from LR express filters. Biologicals 1(49):15–22CrossRef

19.

Wegehaupt AK, Roufs EK, Hewitt CR, Killian ML, Gorbatenko O, Anderson CM et al (2017) Recovery and assessment ofleukocytes from LR express filters. Biologicals 49:15–22CrossRefPubMedPubMedCentral

20.

Peytour Y, Villacreces A, Chevaleyre J, Ivanovic Z, Praloran V (2013) Discarded leukoreduction filters: A new source of stem cells for research, cell engineering, and therapy? Stem Cell Res 11(2):736–742CrossRefPubMed

21.

Teleron AA, Carlson B, Young PP (2005) Blood donor white blood cell reduction filters as a source of human peripheral blood-derived endothelial progenitor cells. Transfusion 45(1):21–25CrossRefPubMed

22.

Weidinger TM, Keller AK, Weiss D, Zimmermann R, Eckstein R, Strasser EF (2011) Peripheral blood mononuclear cells obtained from leukoreduction system chambers show better viability than those from leukapheresis. Transfusion 51(9):2047–2049CrossRefPubMed

23.

Dietz AB, Bulur PA, Emery RL, Winters JL, Epps DE, Zubair AC et al (2006) A novel source of viable peripheral blood mononuclear cells from leukoreduction system chambers. Transfusion 46(12):2083–2089CrossRefPubMed

24.

Izquierdo N, Naranjo M, Fernández M, Cos J, Massuet L, Martínez-Picado J et al (2003) Leukocyte reduction filters: analternative source of peripheral blood mononuclear cells. Inmunología 22(3):255–262

25.

Néron S, Dussault N, Racine C (2006) Whole-blood leukoreduction filters are a source for cryopreserved cells for phenotypic and functional investigations on peripheral blood lymphocytes. Transfusion 46(4):537–544CrossRefPubMed

26.

Ferdowsi S, Pourfathollah AA (2019) Leukocyte reduction filters: a source of peripheral blood leukocytes for research and drug production. Glob J Transfus Med 4:122–123

27.

Ivanovic Z, Duchez P, Morgan DA, Hermitte F, Lafarge X, Chevaleyre J et al (2006) Whole-blood leukodepletion filters as a source of CD34+ progenitors potentially usable in cell therapy. Transfusion 46(1):118–125CrossRefPubMed

28.

Longley RE, Stewart D (1989) Recovery of functional human lymphocytes from leuko-trap filters. J Immunol Methods 121:33–38CrossRefPubMed

29.

Rashidbaigi A, Liao M-J, Hua J, Sidhu M (1999) Recovery of functional human leuko-cytes from recycled filters. Google Patents [US Patent 5,989,441]

30.

Izquierdo N, Naranjo M, Fernández M, Cos J, Massuet L, Martínez-Picado J et al (2003) Leukocyte reduction filters: an alternative source of peripheral blood mononu-clear cells. Inmunología 22:255–262

31.

Bruil A, Beugeling T, Feijen J, van Aken WG (1995) The mechanisms of leukocyte removal by filtration. Transfus Med Rev 9(2):145–166CrossRefPubMed

32.

Dzik S (1993) Leukodepletion blood filters: filter design and mechanisms of leukocyte removal. Transfus Med Rev 7(2):65–77CrossRefPubMed

33.

Kjeldsen- Kragh J, Golebiowska E (2002) Back- priming of the RCM1™ leucocyte- reduction filter: consequences for filtration efficacy. Vox Sang 82(3):127–130CrossRefPubMed

34.

Alici E, Sutlu T, Björkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS (2008) Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components. Blood J Am Soc Hematol 111(6):3155–3162

35.

Destrampe E, Schlueter AJ (2021) Successful autologous peripheral blood stem cell collection using large volume leukapheresis in patients with very low or undetectable peripheral blood CD34+ progenitor cells. Transfus Apheres Sci 60(4):103170CrossRef

36.

Wilde CG, Griffith JE, Marra MN, Snable JL, Scott RW (1989) Purification and characterization of human neutrophil peptide 4, a novel member of the defensin family. J Biol Chem 264(19):11200–11203CrossRefPubMed

37.

Strasser EF, Eckstein R (2010) Optimization of leukocyte collection and monocyte isolation for dendritic cell culture. Transfus Med Rev 24(2):130–139CrossRefPubMed

38.

Larson MC, Hogg N, Hillery CA (2021) Centrifugation removes a population of large vesicles, or “macroparticles”, intermediate in size to RBCs and microvesicles. Int J Mol Sci 22(3):1243CrossRefPubMedPubMedCentral

39.

Wright DG, Kauffmann JC, Chusid MJ, Gallin JI (1975) Functional abnormalities of human neutrophils collected by continuous flow filtration leukapheresis. 901–911

Title: Evaluation of a New Method of Leukocyte Extractions from the Leukoreduction Filter
Authors: Zahra Abbasi Malati
Ali Akbar Pourfathollah
Rasul Dabbaghi
Sahar Balagholi
Mohammad Reza Javan
Publication date: 13-01-2023
Publisher: Springer India
Published in: Indian Journal of Hematology and Blood Transfusion / Issue 3/2023
Print ISSN: 0971-4502
Electronic ISSN: 0974-0449
DOI: https://doi.org/10.1007/s12288-022-01618-x

Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras

Prof. Fabrice Barlesi

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2023

Blood Donation Screening of Transfusion-Transmissible Viral Infection Using Two Different Nucleic Acid Testing (NAT) Platforms: A Single Tertiary Care Oncology Centre Experience

Efficacy and Safety of Biosimilar Romiplostim Versus Innovator Romiplostim in Patients with Chronic Immune Thrombocytopenia

Correction to: Efficacy and Safety of Biosimilar Romiplostim Versus Innovator Romiplostim in Patients with Chronic Immune Thrombocytopenia

Correction to: Efects of AGEs, sRAGE and HMGB1 on Clinical Outcomes in Multiple Myeloma

Comparison of Point-of-Care PT-INR by Hand-Held Device with Conventional PT-INR Testing in Anti-phospholipid Antibody Syndrome Patients on Oral Anticoagulation

The Circular RNA Circ-ANAPC7 as a Biomarker for the Risk Stratification of Myelodysplastic Syndrome

Keynote webinar | Spotlight on antibody–drug conjugates in cancer