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Journal of Cardiovascular Translational Research
Published in: Journal of Cardiovascular Translational Research 1/2021

01-02-2021 | Myocardial Infarction | Original Article

CircMACF1 Attenuates Acute Myocardial Infarction Through miR-500b-5p-EMP1 Axis

Authors: Bo Zhao, Guangping Li, Jianjun Peng, Lihui Ren, Licheng Lei, Huiming Ye, Zuoyan Wang, Sheng Zhao

Published in: Journal of Cardiovascular Translational Research | Issue 1/2021

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Abstract

It is widely accepted that circular RNA (circRNA) plays an important role in cardiovascular diseases. Therefore, this experiment aimed to investigate the pathogenesis of circMACF1 in acute myocardial infarction (AMI). qRT-PCR and immunoblotting were used to detect the expression levels of circMACF1, miR-500b-5p, and epithelial membrane protein 1 (EMP1). The role of circMACF1, miR-500b-5p, and EMP1 in cardiomyocyte apoptosis was assessed using annexin V-FITC/PI. Echocardiographic assessment, serum creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH), myocardial infarct size, and TUNEL staining were applied in our research. In the MI group, the expression levels of circMACF1 and EMP1 were decreased with the increasing expression level of miR-500b-5p. CircMACF1 upregulated the expression of EMP1 as a sponge of miR-500b-5p, and circMACF1 was a direct target of miR-500b-5p. CircMACF1 impaired the progression of AMI by modulating the miR-500b-5p/EMP1 axis. CircMACF1 may be a potential therapeutic target for treating AMI.
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Metadata
Title
CircMACF1 Attenuates Acute Myocardial Infarction Through miR-500b-5p-EMP1 Axis
Authors
Bo Zhao
Guangping Li
Jianjun Peng
Lihui Ren
Licheng Lei
Huiming Ye
Zuoyan Wang
Sheng Zhao
Publication date
01-02-2021
Publisher
Springer US
Published in
Journal of Cardiovascular Translational Research / Issue 1/2021
Print ISSN: 1937-5387
Electronic ISSN: 1937-5395
DOI
https://doi.org/10.1007/s12265-020-09976-5

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