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Journal of Cardiovascular Translational Research

Published in:

01-02-2019 | Original Article

Serum Extracellular Vesicles Retard H9C2 Cell Senescence by Suppressing miR-34a Expression

Authors: Yang Liu, Zhuyuan Liu, Yuan Xie, Cuimei Zhao, Jiahong Xu

Published in: Journal of Cardiovascular Translational Research | Issue 1/2019

Abstract

Extracellular vesicles (EVs) are small-sized membrane-surrounded structures released from cells into the blood, which play important roles in regulating various biological processes. However, the role of EVs in Doxorubicin (DOX)-induced cardiomyocytes senescence remains elusive. In this study, we found that human serum EVs inhibited DOX-induced senescence in H9C2 cells, which was abolished by miR-34a mimic. Our study also proved that miR-34a mediated DOX-induced H9C2 cell senescence by targeting phosphatase 1 nuclear targeting subunit (PNUTS). In addition to the downregulation of miR-34a, EVs could upregulate the expression of PNUTS. Moreover, the inhibitory effect of serum EVs on DOX-induced H9C2 cell senescence was also impeded by PNUTS siRNA. In conclusion, our study suggests that serum EVs retard H9C2 cell senescence through the miR-34a/PNUTS pathway, providing a potential therapy for cardiac aging.

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Title: Serum Extracellular Vesicles Retard H9C2 Cell Senescence by Suppressing miR-34a Expression
Authors: Yang Liu
Zhuyuan Liu
Yuan Xie
Cuimei Zhao
Jiahong Xu
Publication date: 01-02-2019
Publisher: Springer US
Published in: Journal of Cardiovascular Translational Research / Issue 1/2019
Print ISSN: 1937-5387
Electronic ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-018-9847-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Serum Extracellular Vesicles Retard H9C2 Cell Senescence by Suppressing miR-34a Expression

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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