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01-12-2021 | Original Paper

Inhibition of microRNA-143-3p Attenuates Cerebral Ischemia/Reperfusion Injury by Targeting FSTL1

Authors: Shunda Wang, Zhenguo Liu

Published in: NeuroMolecular Medicine | Issue 4/2021

Abstract

MicroRNA (miRNA) miR-143-3p has been reported to participate in the progression of myocardial ischemia/reperfusion (I/R) injury, but its function in cerebral I/R injury remains unclear. Mice were subjected to 60 min of cerebral ischemia followed by different times of reperfusion to construct an I/R injury model in vivo. Human neuroblastoma SH-SY5Y cells were treated with oxygen–glucose deprivation (OGD) for 2 h followed by different times of re-oxygenation to establish I/R injury model in vitro. Neurological deficit was assessed by a five-point score. Infarct volume was detected using 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. The expression of miR-143-3p was evaluated by qRT-PCR. The expression levels of FSTL1, Bcl-2, Bax and cleaved caspase-3 proteins were detected by western blot. The relationship between miR-143-3p and FSTL1 was explored by luciferase reporter assay. Cell viability was measured by CCK-8 assay. Cell apoptosis was evaluated by TUNEL staining and flow cytometry. MiR-143-3p was significantly upregulated during cerebral I/R injury both in vivo and in vitro. Inhibition of miR-143-3p effectively reduced I/R-induced neurological deficit score and infarct volume in vivo, and enhanced cell viability, while decreased cell apoptosis and LDH release of OGD/R-treated SH-SY5Y cells in vitro. Meanwhile, inhibition of miR-143-3p obviously decreased the expression levels of Bax and cleaved caspase-3, while increased the expression levels of Bcl-2. In addition, these changes induced by miR-143-3p inhibition in vitro was effectively reversed by silencing of FSTL1. Our results demonstrated that inhibition of miR-143-3p protected against cerebral I/R injury through targeting FSTL1.

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Title: Inhibition of microRNA-143-3p Attenuates Cerebral Ischemia/Reperfusion Injury by Targeting FSTL1
Authors: Shunda Wang
Zhenguo Liu
Publication date: 01-12-2021
Publisher: Springer US
Published in: NeuroMolecular Medicine / Issue 4/2021
Print ISSN: 1535-1084
Electronic ISSN: 1559-1174
DOI: https://doi.org/10.1007/s12017-021-08650-6

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Inhibition of microRNA-143-3p Attenuates Cerebral Ischemia/Reperfusion Injury by Targeting FSTL1

Abstract

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Abstract

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