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Journal of Gastrointestinal Surgery
Published in: Journal of Gastrointestinal Surgery 6/2010

01-06-2010 | Original Article

Anastomotic Sealing by Extracellular Matrices (ECM) Improves Healing of Colonic Anastomoses in the Critical Early Phase

Authors: Jens Hoeppner, Bettina Wassmuth, Goran Marjanovic, Sylvia Timme, Ulrich Theodor Hopt, Tobias Keck

Published in: Journal of Gastrointestinal Surgery | Issue 6/2010

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Abstract

Background

Extracellular matrices have proven potential for in vivo tissue regeneration at gastrointestinal luminal organs. In this study, small intestinal submucosa (SIS) was tested as a sealant for colonic anastomoses in a rodent model.

Methods

In the rodent model, standard colonic anastomoses in the control group (CG; n = 30) and anastomoses sealed by omentum (n = 30) were compared to SIS-sealed anastomoses in the study group (SG; n = 30). After 4-, 30-, and 90-day macroscopic and microscopic healing, bursting pressure and anastomotic stricture rate were evaluated.

Results

The rate of anastomotic dehiscence was 1/10 after 4 days and 0/10 after 30 and 90 days in all groups. In the SG, the bursting pressure was significantly increased after 4 days compared to CG (148 ± 9 vs. 108 ± 8 mmHg; p > 0.05). Histologically, after 4 days of neovascularization, fibroblast ingrowth and collagen deposition were significantly increased in SG compared to CG. After 30 days, nonsignificant differences were noted in all three parameters. Adhesion rate and anastomotic stricture rate were not significantly affected by SIS sealing after 4, 30, and 90 days.

Conclusion

Especially in the critical phase of anastomotic healing up to day 4, anastomotic healing was improved by SIS sealing. SIS sealing did not cause long-term complications.
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Metadata
Title
Anastomotic Sealing by Extracellular Matrices (ECM) Improves Healing of Colonic Anastomoses in the Critical Early Phase
Authors
Jens Hoeppner
Bettina Wassmuth
Goran Marjanovic
Sylvia Timme
Ulrich Theodor Hopt
Tobias Keck
Publication date
01-06-2010
Publisher
Springer-Verlag
Published in
Journal of Gastrointestinal Surgery / Issue 6/2010
Print ISSN: 1091-255X
Electronic ISSN: 1873-4626
DOI
https://doi.org/10.1007/s11605-010-1191-1

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