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Journal of Gastrointestinal Surgery

Published in:

01-12-2007

Is Autotaxin (ENPP2) the Link between Hepatitis C and Hepatocellular Cancer?

Authors: Amanda B. Cooper, Jianmin Wu, Debao Lu, Mary A. Maluccio

Published in: Journal of Gastrointestinal Surgery | Issue 12/2007

Abstract

Introduction

Hepatitis C is the most significant risk factor for development of hepatocellular carcinoma. Inflammation, fibrosis, and liver cell proliferation may contribute to cancer development either through malignant hepatocyte transformation or extracellular matrix remodeling within the tumor microenvironment. The study objective was to investigate differences in gene expression between patients with Hepatitis C (± cancer) and normal that might explain the increased cancer risk.

Methods

Liver tissue was collected from three patient groups: 1) healthy patients, 2) Hepatitis C patients without cancer, 3) patients with Hepatitis C and hepatocellular carcinoma. Microarray analysis was performed on samples from each group. Western blot and real-time polymerase chain reaction (PCR) analyses corroborated the microarray data. A p value of 0.05 was set as significant.

Results

Microarray analysis showed overexpression of autotaxin in patients with cancer versus hepatitis patients or normal patients. Rho GTPase binding proteins (Cdc42s) associated with lysophosphatidic acid signaling were also overexpressed in cancer patients. Real-time polymerase chain reaction showed overexpression of several factors associated with autotaxin in patients with Hepatitis C (± cancer) versus normal patients.

Conclusions

Patients with Hepatitis C and hepatocellular carcinoma show differential expression of various components of the autotaxin pathway versus normal patients. This merits further investigation in the context of early diagnosis.

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Title: Is Autotaxin (ENPP2) the Link between Hepatitis C and Hepatocellular Cancer?
Authors: Amanda B. Cooper
Jianmin Wu
Debao Lu
Mary A. Maluccio
Publication date: 01-12-2007
Publisher: Springer-Verlag
Published in: Journal of Gastrointestinal Surgery / Issue 12/2007
Print ISSN: 1091-255X
Electronic ISSN: 1873-4626
DOI: https://doi.org/10.1007/s11605-007-0322-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Is Autotaxin (ENPP2) the Link between Hepatitis C and Hepatocellular Cancer?

Abstract

Introduction

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Methods

Results

Conclusions

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