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01-07-2019 | Intoxication | Original Article

Quantification of clitidine in caps and stems of poisonous mushroom Paralepistopsis acromelalga by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Authors: Amin Wurita, Koutaro Hasegawa, Katsuhiro Konno, Kimiko Hashimoto, Kunio Gonmori, Kayoko Minakata, Hideki Nozawa, Itaru Yamagishi, Kanako Watanabe, Osamu Suzuki

Published in: Forensic Toxicology | Issue 2/2019

Abstract

Purpose

A simple and high-throughput analytical method for determining clitidine in Paralepistopsis acromelalga using hydrophilic interaction liquid chromatography–tandem mass spectrometry (LC–MS/MS) was established.

Methods

To determine clitidine in the mushrooms, a simple procedure including dilution with methanol solution and filtering by cartridge was employed just before quantification by LC–MS/MS for high-throughput analysis. In this report, concentrations of clitidine in mushrooms were determined by the standard addition method.

Results

The present established method was successfully applied to the analysis of fruit bodies of P. acromelalga, which were obtained from five different locations in Japan. Results on concentrations of clitidine in each stem and cap of P. acromelalga specimens tested showed that their concentrations were quite different, not only between stems and caps, but also among locations and strains; the concentrations of clitidine in stems and caps ranged from 1.41 to 9.30 mg/g and 3.17 to 14.4 mg/g, respectively.

Conclusions

This is the first report to present a detailed quantitative analysis of clitidine by MS and the distribution of clitidine in stems and caps of P. acromelalga. This analytical method for clitidine was thought to be useful in P. acromelalga poisoning cases to identify the causative toxic mushroom.

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Title: Quantification of clitidine in caps and stems of poisonous mushroom Paralepistopsis acromelalga by hydrophilic interaction liquid chromatography–tandem mass spectrometry
Authors: Amin Wurita
Koutaro Hasegawa
Katsuhiro Konno
Kimiko Hashimoto
Kunio Gonmori
Kayoko Minakata
Hideki Nozawa
Itaru Yamagishi
Kanako Watanabe
Osamu Suzuki
Publication date: 01-07-2019
Publisher: Springer Singapore
Keyword: Intoxication
Published in: Forensic Toxicology / Issue 2/2019
Print ISSN: 1860-8965
Electronic ISSN: 1860-8973
DOI: https://doi.org/10.1007/s11419-019-00470-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Quantification of clitidine in caps and stems of poisonous mushroom Paralepistopsis acromelalga by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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