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Forensic Toxicology
Published in: Forensic Toxicology 1/2015

01-01-2015 | Original Article

Simultaneous determination of prescription drugs, cocaine, aldicarb and metabolites in larvae from decomposed corpses by LC–MS–MS after solid–liquid extraction with low temperature partitioning

Authors: Jéssica de Aguiar França, Marianna Brandão, Fernando Fabriz Sodré, Eloisa Dutra Caldas

Published in: Forensic Toxicology | Issue 1/2015

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Abstract

The determination of toxic substances in insect larvae collected from corpses in an advanced state of putrefaction can help to elucidate the causa mortis in forensic cases. In this study, an analytical method for the simultaneous determination of six prescription drugs, cocaine and its metabolite benzoylecgonine, and aldicarb and its sulfone and sulfoxide metabolites in larvae was developed and validated. The method involved a solid–liquid extraction with low temperature partitioning, and determination by a liquid chromatography–mass spectrometry (LC–MS–MS) method. Significant matrix effects were observed for most analytes, indicating the need to use analytical curves in pre-extracted fortified matrices. The limits of quantification ranged from 1 to 40 ng/g, with precision between 2.83 and 16.9 %. The validated method was used to analyze 28 actual larval samples collected from corpses at the Forensic Medical Institute between 2009 and 2012 in the Federal District, Brazil. At least one substance was present in 11 samples. Benzoylecgonine and diazepam were found in four samples each, followed by cocaine (three samples), carbamazepine (two samples) and amitriptyline (one sample). The method proved to be simple, fast and of low cost, and can be used by forensic laboratories as a complementary tool to elucidate intoxication-related deaths where usual toxicological matrices are not available.
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Metadata
Title
Simultaneous determination of prescription drugs, cocaine, aldicarb and metabolites in larvae from decomposed corpses by LC–MS–MS after solid–liquid extraction with low temperature partitioning
Authors
Jéssica de Aguiar França
Marianna Brandão
Fernando Fabriz Sodré
Eloisa Dutra Caldas
Publication date
01-01-2015
Publisher
Springer Japan
Published in
Forensic Toxicology / Issue 1/2015
Print ISSN: 1860-8965
Electronic ISSN: 1860-8973
DOI
https://doi.org/10.1007/s11419-014-0255-4

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