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01-07-2013 | Original Paper

Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells

Authors: Kanuengnit Choochuay, Preedakorn Chunhacha, Varisa Pongrakhananon, Rataya Luechapudiporn, Pithi Chanvorachote

Published in: Journal of Natural Medicines | Issue 3/2013

Abstract

The anoikis-sensitization activity of imperatorin, an active furanocoumarin component of Angelica dahurica root, is reported herein for the first time. The present study demonstrated that the imperatorin treatment at sub-toxic concentrations enhanced human lung cancer H23 cell apoptosis after detachment. A Western blot analysis showed that imperatorin significantly enhanced the p53 protein level, which subsequently down-regulated Mcl-1 protein and up-regulated Bax, while it had a minimal effect on Bcl-2 expression. In addition, an anchorage-independent growth assay was performed to support the anti-metastasis potential of imperatorin. Consistent with anoikis assay, imperatorin exhibited a strong inhibitory effect on the anchorage-independent growth of the cells. Further, this study demonstrated that imperatorin sensitizes anoikis in other lung cancer cells, namely, H292 and A549. Because anoikis was shown to be a critical hindrance in preventing cancer cell metastasis, the knowledge regarding such an activity and an underlying mechanism may lead to the development of this compound for a cancer therapy.

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Title: Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells
Authors: Kanuengnit Choochuay
Preedakorn Chunhacha
Varisa Pongrakhananon
Rataya Luechapudiporn
Pithi Chanvorachote
Publication date: 01-07-2013
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 3/2013
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-012-0719-y

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Imperatorin sensitizes anoikis and inhibits anchorage-independent growth of lung cancer cells

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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