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01-07-2013 | Note

Anti-degranulating activity in rat basophil leukemia RBL-2H3 cells of flavanone glycosides and their aglycones in citrus fruits

Authors: Kazuya Murata, Seiya Takano, Megumi Masuda, Munekazu Iinuma, Hideaki Matsuda

Published in: Journal of Natural Medicines | Issue 3/2013

Abstract

The anti-degranulating activity of flavonoids present in Citrus fruits was comprehensively evaluated. Among these, hesperetin and naringenin, respectively aglycones of hesperidin and narirutin, showed significant activity. The targets of hesperetin and naringenin were found: hesperetin inhibited phosphorylation of Syk and Akt, while naringenin suppressed the expression of Lyn and inhibited the phosphorylation of Akt. These results suggest that hesperetin and naringenin inhibit degranulation by suppression of pathway signals and reduce the symptoms of allergy by inhibiting phosphorylation of Akt, which leads to the suppression of cytokines. In addition, hesperetin showed inhibitory activity against the degranulation induced by calcium ionophores, indicating that hesperetin exerts its inhibitory activity by stabilizing the membrane structure.

Kubo M, Yano M, Matsuda H (1989) Pharmacological study on Citrus fruits. I. Anti-allergic effect of fruit of Citrus unshiu Markovich (1). Yakugaku Zasshi 109:835–842PubMed

Matsuda H, Yano M, Kubo M, Iinuma M, Oyama M, Mizuno M (1991) Pharmacological study on Citrus fruits. II. Anti-allergic effect of fruit of Citrus unshiu Markovich (2). On flavonoid components. Yakugaku Zasshi 111:193–198PubMed

Kuroyanagi M, Ishii H, Kawahara N, Sugimoto H, Yamada H, Okihara K, Shirota O (2008) Flavonoid glycosides and limonoids from Citrus molasses. J Nat Med 62:107–111PubMedCrossRef

Fujita T, Shiura T, Masuda M, Tokunaga M, Kawase A, Iwaki M, Gato T, Fumuro M, Sasaki K, Utsunomiya N, Matsuda H (2008) Anti-allergic effect of a combination of Citrus unshiu unripe fruits extract and prednisolone on picryl chloride-induced contact dermatitis in mice. J Nat Med 62:202–206PubMedCrossRef

Zheng C, Lu Y, Tao L, Tao X, Su X, Wei D (2007) Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts. J Enzyme Inhib Med Chem 22:91–98CrossRef

Itoh K, Masuda M, Naruto S, Murata K, Matsuda H (2009) Antiallergic activity of unripe Citrus hassaku fruits extract and its flavanone glycosides on chemical substance-induced dermatitis in mice. J Nat Med 63:443–450PubMedCrossRef

Itoh K, Hirata N, Masuda M, Naruto S, Murata K, Wakabayashi K, Matsuda H (2009) Inhibitory effects of Citrus hassaku extract and its flavanone glycosides on melanogenesis. Biol Pharm Bull 32:410–415PubMedCrossRef

Fujita T, Kawase A, Nishijima N, Masuda M, Matsuda H, Iwaki M (2008) Gastrointestinal absorption of hesperidin and hesperetin after oral administration of immature Citrus unshiu extract to rats. Shoyakugaku Zasshi 62:8–14

Kubo M, Fujita T, Nishimura S, Tokunaga M, Matsuda H, Gato T, Tomohiro N, Sasaki K, Utsunomiya N (2004) Seasonal variation in anti-allergic activity of Citrus fruits and flavanone glycoside content. Nat Med 58:284–294

10.

Matsumoto H, Ikoma Y, Sugiura M, Yano M, Hasegawa Y (2004) Identification and quantification of the conjugated metabolites derived from orally administered hesperidin in rat plasma. J Agric Food Chem 52:6653–6659PubMedCrossRef

11.

Kobayashi S, Tanabe S (2006) Evaluation of the anti-allergic activity of Citrus unshiu using rat basophilic leukemia RBL-2H3 cells as well as basophils of patients with seasonal rhinitis to pollen. Int J Mol Med 17:511–515PubMed

12.

Tordera M, Ferrandiz ML, Alcaraz MJ (1994) Influence of anti-inflammatory flavonoids on degranulation and arachidonic acid release in rat neutrophils. Z Naturforsch 49:235–240

13.

Shichijo M, Yamamoto N, Tsujishita H, Kimata M, Nagai H, Kokubo T (2003) Inhibition of Syk activity and degranulation of human mast cell by flavonoids. Biol Pharm Bull 26:1685–1690PubMedCrossRef

14.

Higashijima T, Burnier J, Ross E (1990) Regulation of G_i and G_o by mastoparan, related amphiphilic peptides, and hydrophobic amines. J Biol Chem 265:14176–14186PubMed

Title: Anti-degranulating activity in rat basophil leukemia RBL-2H3 cells of flavanone glycosides and their aglycones in citrus fruits
Authors: Kazuya Murata
Seiya Takano
Megumi Masuda
Munekazu Iinuma
Hideaki Matsuda
Publication date: 01-07-2013
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 3/2013
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-012-0699-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Anti-degranulating activity in rat basophil leukemia RBL-2H3 cells of flavanone glycosides and their aglycones in citrus fruits

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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